J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jmm.0.012856-0v1
58/12/1611    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Chitambar, S. D.
Right arrow Articles by Chhabra, P.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chitambar, S. D.
Right arrow Articles by Chhabra, P.
Agricola
Right arrow Articles by Chitambar, S. D.
Right arrow Articles by Chhabra, P.
J Med Microbiol 58 (2009), 1611-1615; DOI: 10.1099/jmm.0.012856-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Molecular characterization of a rare G1P[19] rotavirus strain from India: evidence of reassortment between human and porcine rotavirus strains

Shobha D. Chitambar, Ritu Arora and Preeti Chhabra

Enteric Viruses Department, National Institute of Virology, Pune 411001, India

Correspondence
Shobha D. Chitambar
chitambars{at}hotmail.com

Received May 14, 2009
Accepted August 9, 2009

This study pertains to the characterization of a human rotavirus strain (NIV929893) with a rare specificity of G1P[19]. Three structural genes (VP4, VP6 and VP7) and one non-structural gene (NSP4) of strain NIV929893 were subjected to RT-PCR for amplification of entire coding regions. All of the amplicons were sequenced to carry out phylogenetic analysis. The complete amino acid sequences of the VP7 and VP4 gene products showed clustering of the VP7 gene with G1 strains of human origin and the VP4 gene with P[19] strains of porcine origin. The two viral proteins VP6 and NSP4, described previously as genetically linked proteins, were shown to be subgroup II and genotype B of human and porcine origins, respectively. The findings of this study provide evidence of reassortment between VP7/VP6 genes of humans and VP4/NSP4 genes of porcine species and an independent segregation of VP6 and NSP4 genes in a group A human rotavirus strain with G1P[19] specificity.

The GenBank/EMBL/DDBJ accession numbers for the sequences determined in this study are DQ886957 (VP7), DQ887060 (VP4), FJ685614 (VP6) and FJ685615 (NSP4).






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.