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J Med Microbiol 58 (2009), 1433-1436; DOI: 10.1099/jmm.0.012203-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Rapid detection of anti-hepatitis A virus neutralizing antibodies in a microplate enzyme immunoassay

Ali Azizi1,2,3, Danylo Sirskyj1, Richard Weltzin1, David E. Anderson1 and Francisco Diaz-Mitoma1,2,3

1 Variation Biotechnologies Inc., 1740 Woodroffe Avenue, Building 400, Ottawa, ON K2G 3R8, Canada

2 Infectious Disease and Vaccine Research Center, Children's Hospital of Eastern Ontario Research Institute, 401 Smyth, Ottawa, ON K1H 8L1, Canada

3 Department of Pathology and Laboratory Medicine, University of Ottawa, 451 Smyth, Ottawa, ON K1H 8M5, Canada

Correspondence
Ali Azizi
aazizi{at}uottawa.ca

Received April 24, 2009
Accepted July 13, 2009

The slow growth of hepatitis A virus (HAV) in cell culture is one of the primary pitfalls in the development of sensitive and rapid methods for the detection and quantification of HAV and associated neutralizing antibodies. Currently, in vitro assays frequently require 8 days or more to detect and quantify the presence of HAV neutralizing antibodies. This study describes a rapid immunoassay that allowed the detection of anti-HAV neutralizing antibodies in only 3 days. This microplate-based enzymic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of HAV vaccines and in quantifying neutralizing antibodies during the course of HAV infection.






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