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J Med Microbiol 58 (2009), 65-68; DOI: 10.1099/jmm.0.47707-0
© 2009 Society for General Microbiology
ISSN 0022-2615

Molecular diagnosis of Kingella kingae osteoarticular infections by specific real-time PCR assay

Abdessalam Cherkaoui1, Dimitri Ceroni2, Stéphane Emonet1, Yan Lefevre2 and Jacques Schrenzel1,3

1 Clinical Microbiology Laboratory, Service of Infectious Diseases, University of Geneva Hospitals (HUG), CH-1211 Geneva 14, Switzerland

2 Pediatric Orthopedic Service, University of Geneva Hospitals (HUG), CH-1211 Geneva 14, Switzerland

3 Genomic Research Laboratory, Service of Infectious Diseases, University of Geneva Hospitals (HUG), CH-1211 Geneva 14, Switzerland

Correspondence
Jacques Schrenzel
jacques.schrenzel{at}genomic.ch

Received October 19, 2007
Accepted August 28, 2008

Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene and illustrate its use in two clinical cases. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no cross-reactivity with several related species and common osteoarticular pathogens.




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B. Ilharreborde, P. Bidet, M. Lorrot, J. Even, P. Mariani-Kurkdjian, S. Liguori, C. Vitoux, Y. Lefevre, C. Doit, F. Fitoussi, et al.
New Real-Time PCR-Based Method for Kingella kingae DNA Detection: Application to Samples Collected from 89 Children with Acute Arthritis
J. Clin. Microbiol., June 1, 2009; 47(6): 1837 - 1841.
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