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The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice

¹ State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia

² Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Pettenkofer Str. 9a, 80336 Munich, Germany

³ Canadian Food Inspection Agency Lethbridge Laboratory, PO 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada

Correspondence
Andrey P. Anisimov
anisimov{at}obolensk.org

Received August 5, 2008
Accepted September 17, 2008

Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6^– mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naïve mice or animals immunized with this antigen.

Abbreviations: Ap^R, ampicillin-resistant; Ap^S, ampicillin-sensitive; BHI, brain heart infusion; Cm^R, chloramphenicol-resistant; i.p., intraperitoneal; i.v., intravenous; Km^R, kanamycin-resistant; Pm^R, polymixin B-resistant; s.c., subcutaneous; Suc^R, sucrose-resistant; Tc^R, tetracycline-resistant; TSISCBP, Tarasevich State Institute for Standardization and Control of Biomedical Preparations.

The GenBank/EMBL/DDBJ accession number for the determined nucleotide sequence of the psa operon of Y. pestis strain 231 is EF079883.

A supplementary table of primer sequences is available with the online version of this paper.

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