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J Med Microbiol 57 (2008), 1152-1156; DOI: 10.1099/jmm.0.47827-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to feline corneocytes

Aline Baldo1, Jérémy Tabart1, Sandy Vermout2, Anne Mathy1, Alfred Collard3, Bertrand Losson1 and Bernard Mignon1

1 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, B43 Sart Tilman, 4000 Liège, Belgium

2 Federal Agency for Medicinal and Health Products, Victor Horta Plein 40/40, 1060 Brussels, Belgium

3 Centre d'Economie Rurale (CER), Animal Immunology, Rue du Carmel 1, 6900 Marloie, Belgium

Correspondence
Bernard Mignon
bmignon{at}ulg.ac.be

Received 18 December 2007
Accepted 7 May 2008

Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis, mainly in cats and humans. The mechanisms involved in adherence of M. canis to epidermis have never been investigated. Here, a model was developed to study the adherence of M. canis to feline corneocytes through the use of a reconstructed interfollicular feline epidermis (RFE). In this model, adherence of arthroconidia to RFE was found to be time-dependent, starting at 2 h post-inoculation and still increasing at 6 h. Chymostatin, a serine protease inhibitor, inhibited M. canis adherence to RFE by 53 %. Moreover, two mAbs against the keratinolytic protease subtilisin 3 (Sub3) inhibited M. canis adherence to RFE by 23 %, suggesting that subtilisins, and Sub3 in particular, are involved in the adherence process.

Abbreviations: AAPF-pNa, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide; RFE, reconstructed interfollicular feline epidermis.






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