J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
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J Med Microbiol 57 (2008), 954-956; DOI: 10.1099/jmm.0.46992-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Agar dilution and agar screen with cefoxitin and oxacillin: what is known and what is unknown in detection of meticillin-resistant Staphylococcus aureus

Leandro Reus Rodrigues Perez1,2, Cícero Dias2,3 and Pedro Alves d'Azevedo1,3

1 Post-Graduate Program in Medical Sciences, Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, RS, Brazil

2 Laboratory of Microbiology, Hospital Mãe de Deus, Porto Alegre, RS, Brazil

3 Microbiology Department, Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, Porto Alegre, RS, Brazil

Correspondence
Leandro Reus Rodrigues Perez
leandro.reus{at}gmail.com

Received 7 October 2006
Accepted 4 April 2008

In this study we evaluated the performance of the oxacillin agar screen test, and agar dilution tests using cefoxitin and oxacillin antimicrobials, to detect meticillin resistance in Staphylococcus aureus isolates. The presence of the mecA gene, detected by PCR, was used as the standard to which agar screen and agar dilution tests were compared. The best performance was obtained using the agar dilution test (99.4 % accuracy) with breakpoints of 4 µg ml–1 for oxacillin and 8 µg ml–1 for cefoxitin, and using the oxacillin agar screen test. Also, a strong correlation between MIC values of cefoxitin and oxacillin permits the use of either drug for detection of meticillin resistance.

Abbreviations: CLSI, Clinical and Laboratory Standards Institute; MRSA, meticillin-resistant S. aureus; PBP, penicillin-binding protein.






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