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J Med Microbiol 57 (2008), 1024-1027; DOI: 10.1099/jmm.0.2008/000406-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods: resazurin and nitrate reductase assays

Dissou Affolabi1,2, N'Dira Sanoussi1, Mathieu Odoun1, Anandi Martin2,3, Louis Koukpemedji1, Juan Carlos Palomino2, Luc Kestens4, Séverin Anagonou1 and Françoise Portaels2

1 Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin

2 Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium

3 Médecins Sans Frontières, 75011 Paris, France

4 Immnunology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium

Correspondence
Dissou Affolabi
affolabi_dissou{at}yahoo.fr

Received 14 January 2008
Accepted 23 April 2008

We have evaluated two simple, rapid and low-cost colorimetric methods for the detection of multidrug-resistant Mycobacterium tuberculosis. A total of 151 M. tuberculosis strains were tested for resistance to rifampicin (RMP) and isoniazid by resazurin microplate assay (REMA) and nitrate reductase assay (NRA) in comparison with the conventional proportion method (PM) on Löwenstein-Jensen medium. A complete agreement was found between NRA and PM, while one false RMP-susceptible result was found by REMA. REMA and NRA tests are rapid and inexpensive, and could be good alternatives to the conventional PM in low-resource countries.

Abbreviations: INH, isoniazid; NRA, nitrate reductase assay; PM, proportion method; REMA, resazurin microplate assay; RMP, rifampicin; TB, tuberculosis; WHO, World Health Organization.






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