J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Taillon, C.
Right arrow Articles by Dubreuil, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taillon, C.
Right arrow Articles by Dubreuil, J. D.
Agricola
Right arrow Articles by Taillon, C.
Right arrow Articles by Dubreuil, J. D.
J Med Microbiol 57 (2008), 887-890; DOI: 10.1099/jmm.0.2008/000281-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Heterogeneity of Escherichia coli STb enterotoxin isolated from diseased pigs

Christine Taillon, Eric Nadeau, Michaël Mourez and J. Daniel Dubreuil

Centre de Recherche en Infectiologie Porcine (CRIP), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 7C6, Canada

Correspondence
J. Daniel Dubreuil
daniel.dubreuil{at}umontreal.ca

Received 9 January 2008
Accepted 16 March 2008

To investigate the presence and frequency of estB variant(s), a collection of 100 STb-positive enterotoxigenic Escherichia coli (ETEC) strains isolated from 1980 to 2007 inclusively and randomly selected from diseased pigs in Québec, Canada, was analysed. A wide diversity of virulence gene profiles (virotypes) was detected in the strain collection. The estB gene was amplified by PCR using primers designed from the signal sequence and the C-terminal end, and the amplified fragment was sequenced using the forward primer. The translated DNA sequence revealed a His12->Asn change in 23 of the 100 ETEC isolates tested. The STb-variant strains were observed throughout the sampling period covered in the study. No other STb-variant type was found in this study. All 23 variant strains were also positive for the STa enterotoxin and were resistant to tetracycline, as for strain 2173. The STb variant was associated with Stx2-positive strains (5/6) and STa : STb strains that did not harbour any of the tested porcine fimbrial adhesins (13/17). The remaining variant strains were associated with fimbriae F4 (1/40), F5 (1/6), F6 (1/1) and F18 (2/7; excluding F18 : Stx2 strains).

Abbreviations: AIDA, adhesin involved in diffuse adherence; EAE, E. coli attaching-and-effacing factor; EAST1, enteroaggregative E. coli heat-stable enterotoxin 1; ETEC, enterotoxigenic Escherichia coli; LT, heat-labile toxin; Paa, porcine attaching- and effacing-associated protein; ST, heat-stable enterotoxin; Stx, Shiga toxin.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2008 Society for General Microbiology.