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J Med Microbiol 57 (2008), 873-880; DOI: 10.1099/jmm.0.47564-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Promising loci of variable numbers of tandem repeats for typing Beijing family Mycobacterium tuberculosis

Yoshiro Murase, Satoshi Mitarai, Isamu Sugawara, Seiya Kato and Shinji Maeda

Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan

Correspondence
Shinji Maeda
maeda{at}jata.or.jp

Received 8 August 2007
Accepted 13 March 2008

We analysed the genotypes of 325 Mycobacterium tuberculosis clinical isolates obtained during 2002 throughout Japan. The genotyping methods included insertion sequence IS6110 RFLP, spoligotyping and variable number of tandem repeat (VNTR) analyses. Clustered isolates revealed by IS6110 RFLP analysis accounted for 18.5 % (60/325) of the isolates. Beijing genotype tuberculosis (TB) accounted for 73.8 % (240/325) of the isolates. Using VNTR, we analysed 35 loci, including 12 standard mycobacterial interspersed repetitive units and 4 exact tandem repeats. The discriminatory power of these 16 loci was low. Using VNTR analyses of the 35 loci, 12 loci (VNTRs 0424, 0960, 1955, 2074, 2163b, 2372, 2996, 3155, 3192, 3336, 4052 and 4156) were selected for the genotyping of Beijing genotype strains. Comparison of the discriminatory power of the 12-locus VNTR [Japan Anti-Tuberculosis Association (JATA)] to that of the 15-locus and 24-locus VNTRs proposed by Supply et al. (2006) showed that our established VNTR system was superior to the reported 15-locus VNTR and had almost equal discriminatory power to the 24-locus VNTR. This 12-locus VNTR (JATA) can therefore be used for TB genotyping in areas where Beijing family strains are dominant.

Abbreviations: CDC, Centers for Disease Control and Prevention; ETR, exact tandem repeat; HGDI, Hunter–Gaston discriminatory index; JATA, Japan Anti-Tuberculosis Association; MIRU, mycobacterial interspersed repetitive unit; TB, tuberculosis; VNTR, variable number of tandem repeats.






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