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J Med Microbiol 57 (2008), 803-808; DOI: 10.1099/jmm.0.47789-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Biochemical properties and primary structure of elastase inhibitor AFUEI from Aspergillus fumigatus

Yoshiyuki Okumura1,2, Takeshi Matsui2, Kenji Ogawa3, Kei-ichi Uchiya2 and Toshiaki Nikai2

1 Department of Validation, Matsuurayakugyo Co. Ltd, 24-21 Enjo-chou, Syowa-ku, Nagoya, Aichi 466-0054, Japan

2 Department of Microbiology, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya, Aichi 468-8503, Japan

3 Department of Pulmonary Medicine, Higashi Nagoya National Hospital, 5-101 Umemorizaka, Meito-ku, Nagoya, Aichi 465-8620, Japan

Correspondence
Yoshiyuki Okumura
kinyu{at}gld.mmtr.or.jp

Received 30 November 2007
Accepted 28 February 2008

An elastase inhibitor from Aspergillus fumigatus (AFUEI) was isolated, and its biochemical properties and primary structure examined. The inhibitor was purified by column chromatography using DE52 cellulose and Sephadex G-75, and was found to be homogeneous as indicated by a single band following discontinuous PAGE and SDS-PAGE. A molecular mass of 7525.1 Da was observed by matrix-assisted desorption/ionization time-of-flight mass spectroscopy. The elastolytic activity of elastases from A. fumigatus, Aspergillus flavus and human leukocytes was inhibited by AFUEI. However, the elastolytic activity of porcine pancreas elastase, Pseudomonas aeruginosa elastase and elastase from snake venom was not affected by AFUEI. No inhibitory effect of DTT or 2-mercaptoethanol on the elastase inhibitory activity of AFUEI was observed. The amino acid sequence of AFUEI peptides derived from digests utilizing clostripain was determined by Edman sequencing. AFUEI was composed of 68 aa and had a calculated molecular mass of 7526.2 Da. The search for amino acid homology with other proteins demonstrated that aa 1–68 of AFUEI are 100 % identical to aa 20–87 of the hypothetical protein AFUA 3G14940 of A. fumigatus.

Abbreviations: GAAPLNA, glutaryl-L-alanyl-L-alanyl-L-proryl-L-leucine p-nitroanilide; MALDI/TOF-MS, matrix-assisted desorption/ionization time-of-flight mass spectroscopy; Pe-AFUEI, S-pyridylethylated AFUEI; pNA, p-nitroanilide.






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