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J Med Microbiol 57 (2008), 658-663; DOI: 10.1099/jmm.0.47677-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Case Report

Severe course of rat bite-associated Weil's disease in a patient diagnosed with a new Leptospira-specific real-time quantitative LUX-PCR

Alexandra Roczek1, Christian Forster2, Heribert Raschel1, Stefan Hörmansdorfer1, Karl-Heinz Bogner1, Angela Hafner-Marx1, Hans Lepper1, Gerhard Dobler3, Mathias Büttner1,{dagger} and Andreas Sing1,{dagger}

1 Bavarian Health and Food Safety Authority (LGL), Veterinärstraße 2, 85764 Oberschleißheim, Germany

2 Universitätsklinikum Erlangen, Medizinische Klinik 4, Krankenhausstraße 12, 91054 Erlangen, Germany

3 Institute of Microbiology of the Bundeswehr, Neuherbergstraße 11, 80937 München, Germany

Correspondence
Andreas Sing
andreas.sing{at}lgl.bayern.de

Received 4 October 2007
Accepted 9 January 2008

Leptospirosis is a zoonotic disease with global distribution, caused by spirochaetes of the genus Leptospira. Transmission of Leptospira interrogans serovar Icterohaemorrhagiae, the causative agent of Weil's disease, to humans usually results from exposure to the urine of infected, but mostly asymptomatic, rodents, either by direct contact or indirectly through contaminated soil or water. Although regarded as a re-emerging infectious disease, human leptospirosis is probably underdiagnosed due to its often unspecific clinical appearance and difficulties in culturing leptospires. Therefore, more rapid and specific diagnostic procedures are needed. Here we describe a novel real-time quantitative PCR system developed for the accurate and fast diagnosis of pathogenic Leptospira spp. Its usefulness in the management of a patient with rat bite-associated multiorgan failure is demonstrated.

Abbreviations: Ct, threshold cycle; FAM, 6-carboxyfluorescein; LUX, Light Upon eXtension; MAT, microscopic agglutination test; qPCR, quantitative PCR; Tm, melting temperature.

{dagger}These authors contributed equally to this work.




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K. Lourdault, F. Aviat, and M. Picardeau
Use of quantitative real-time PCR for studying the dissemination of Leptospira interrogans in the guinea pig infection model of leptospirosis
J. Med. Microbiol., May 1, 2009; 58(5): 648 - 655.
[Abstract] [Full Text] [PDF]


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