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J Med Microbiol 57 (2008), 585-591; DOI: 10.1099/jmm.0.47783-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide

Eleonora Altman1, Heriberto Fernández2, Vandana Chandan1, Blair A. Harrison1, Myra Wilson Schuster2, Laura Otth Rademacher2 and Claudio Toledo3

1 Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada

2 Instituto de Microbiología Clínica, Edificio de Ciencias Biomédicas, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile

3 Instituto de Medicina, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile

Correspondence
Eleonora Altman
Eleonora.altman{at}nrc-cnrc.gc.ca

Received 27 November 2007
Accepted 19 January 2008

Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Lex) and Ley epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Lea and Leb structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Lex and/or Ley epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Leb blood group determinants, in addition to type 2 Lex and Ley structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %).

Abbreviations: CagA, cytotoxin-associated antigen; HRP, horseradish peroxidase; Le, Lewis; WCE, whole-cell indirect ELISA.




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B.-S. Sheu and J.-J. Wu
Type 1 and 2 Lewis antigens of Helicobacter pylori - a potential marker of the human geographical distribution
J. Med. Microbiol., May 1, 2008; 57(5): 543 - 544.
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