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J Med Microbiol 57 (2008), 581-584; DOI: 10.1099/jmm.0.47455-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Mycobacterium tuberculosis co-existence with humans: making an imprint on the macrophage P2X7 receptor gene?

Igor Mokrousov1, Nadezhda Sapozhnikova2 and Olga Narvskaya1

1 Laboratory of Molecular Microbiology, St Petersburg Pasteur Institute, St Petersburg 197101, Russia

2 Research Institute of Phthisiopulmonology, St Petersburg 193063, Russia

Correspondence
Igor Mokrousov
imokrousov{at}mail.ru
or
igormokrousov{at}yahoo.com

Received 18 June 2007
Accepted 11 January 2008

The development of tuberculosis (TB) infection in humans depends on the mycobacterial strain and the human host, and is multigenically controlled in both. ATP ligation of P2X7 receptors expressed on human macrophages infected with mycobacteria induces cell death and subsequent loss of intracellular bacterial viability. This study analysed the allelic distribution of two single-nucleotide polymorphisms (SNPs) in the P2RX7 gene in the Slavic population of the St Petersburg area of Russia. Analysis of the –762 C/T P2RX7 promoter SNP revealed no significant association between pulmonary TB patients and control subjects (3x2 {chi}2=3.2, 1 d.f., P=0.2). The –762C allele was highly and almost equally represented in both groups in this study (68.2 % in patients and 69.3 % in controls). This result differs strikingly from a Gambian study where this allele was found in only 7 and 12 % of pulmonary TB patients and controls, respectively [Li, C. M., Campbell, S. J., Kumararatne, D. S., Bellamy, R., Ruwende, C., McAdam, K. P. W. J., Hill, A. V. S. & Lammas, D. A. (2002). J Infect Dis 186, 1458–1462]. In contrast, the frequency of the C allele at position 1513 in exon 13, resulting in a loss of P2X7 function, was significantly higher among pulmonary TB patients in this study (P=0.02). Thus, analysis of the P2X7 receptor gene in the Russian Slavic population showed that the 1513C allele, acting dominantly, is a possible risk factor for clinical TB, whereas the –762 P2RX7 polymorphism did not appear to be associated with human susceptibility to TB.

Abbreviations: CI, confidence interval; HIV, human immunodeficiency virus; HWE, Hardy–Weinberg equilibrium; LD, linkage disequilibrium; OR, odds ratio; SNP, single-nucleotide polymorphism; TB, tuberculosis.

A table showing the results of stratification analysis for the –762 and 1513 genotypes and alleles is available as supplementary material with the online version of this paper.






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