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J Med Microbiol 57 (2008), 570-580; DOI: 10.1099/jmm.0.47803-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Identification of hyperinvasive Campylobacter jejuni strains isolated from poultry and human clinical sources

Catherine Fearnley1, Georgina Manning2, Mary Bagnall1, Muhammad Afzal Javed2, Trudy M. Wassenaar3 and Diane G. Newell1

1 Veterinary Laboratories Agency (Weybridge), Surrey KT15 3NB, UK

2 School of Science and Technology, Nottingham Trent University, NG11 8NS, UK

3 Molecular Microbiology and Genomics Consultants, Zotzenheim, Germany

Correspondence
Diane G. Newell
d.newell{at}vla.defra.gsi.gov.uk

Received 7 December 2007
Accepted 29 January 2008

Campylobacter jejuni causes gastroenteritis with a variety of symptoms in humans. In the absence of a suitable animal model, in vitro models have been used to study virulence traits such as invasion and toxin production. In this study, 113 C. jejuni isolates from poultry and poultry-related (n=74) environments as well as isolates from human cases (n=39) of campylobacteriosis and bacteraemia were tested for invasiveness using INT 407 cells. The method was sufficiently reproducible to observe a spectrum of invasiveness amongst strains. As a result, strains were classified as low, high and hyper-invasive. The majority of strains (poultry and human) were low invaders (82 % and 88 %, respectively). High invasion was found for 5 % of human strains and 11 % of poultry-related isolates. However, only 1 % of poultry strains were classified as hyperinvasive compared to 13 % of human isolates (P=0.0182). Of those isolates derived from the blood of bacteraemic patients, 20 % were hyperinvasive, though this correlation was not statistically significant. An attempt was made to correlate invasiveness with the presence of seven genes previously reported to be associated with virulence. Most of these genes did not correlate with invasiveness, but gene cj0486 was weakly over-represented, and a negative correlation was observed for the gene ciaB. This trend was stronger when the two genes were analysed together, thus ciaB– cj0486+ was over-represented in high and hyperinvasive strains, with low invaders more commonly found to lack these genes (P=0.0064).

Abbreviations: MLST, multilocus sequence typing; ST, sequence type.




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