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J Med Microbiol 57 (2008), 469-475; DOI: 10.1099/jmm.0.47634-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Genotyping analysis of Mycoplasma pneumoniae clinical strains in Japan between 1995 and 2005: type shift phenomenon of M. pneumoniae clinical strains

Tsuyoshi Kenri1, Norio Okazaki2, Tsutomu Yamazaki3, Mitsuo Narita4, Kinich Izumikawa5, Mayumi Matsuoka1, Satowa Suzuki1, Atsuko Horino1,6 and Tsuguo Sasaki1

1 Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan

2 Kanagawa Prefectural Institute of Public Health, Chigasaki, Kanagawa 253-0087, Japan

3 Department of Pediatrics, Saitama Medical School, Iruma, Saitama 350-0495, Japan

4 JR Sapporo Tetsudo Hospital, Sapporo, Hokkaido 060-0033, Japan

5 Izumikawa Hospital, Minamitakaki, Nagasaki 859-1504, Japan

6 Graduate School of Health Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-0034, Japan

Correspondence
Tsuyoshi Kenri
kenri{at}nih.go.jp

Received 15 September 2007
Accepted 4 December 2007

Mycoplasma pneumoniae clinical isolates obtained between 1995 and 2005 were examined to determine the prevalent genotype. One hundred and twenty-seven strains isolated from bronchitis and pneumonia patients were genotyped by a PCR-RFLP method based on nucleotide sequence polymorphisms of the p1 gene, which encodes the major adhesin protein. The typing results established that 66 of the isolates were group I strains, 45 were group II strains and 16 were group II variants. Analysis of the annual occurrence of these isolates showed a predominance of group II strains between 1995 and 2001 (n=37). No group I strain was found during this period. However, group I strains appeared in the isolates from 2002 (2/5 isolates, 40 %) and increased in specimens taken after 2003, thereby constituting a large proportion of the isolates. In 2004 and 2005, no group II strains were found among the isolates (n=49), although there were nine group II variants. Throat swabs and sputum samples obtained from patients with respiratory infections between 1997 and 2005 were also analysed by PCR-RFLP or a new nested PCR to detect the p1 gene DNA. Typing analysis of these p1 gene DNAs also showed that the group I p1 gene was not present in specimens taken before 2000, but was present and dominant in specimens taken after 2001. These results indicate that, in Japan, the prevalent type of M. pneumoniae changed from a group II strain to a group I strain around 2002.

Abbreviations: HA, haemadsorption.






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