J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
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J Med Microbiol 57 (2008), 444-451; DOI: 10.1099/jmm.0.47688-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Development and evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Campylobacter jejuni and Campylobacter coli

Wataru Yamazaki1, Masumi Taguchi1, Masanori Ishibashi1, Miyoshi Kitazato2, Masafumi Nukina3, Naoaki Misawa4 and Kiyoshi Inoue1

1 Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan

2 Yodogawa Christian Hospital, Osaka, Japan

3 Microbiological Department, Kobe Institute of Health, Hyogo, Japan

4 Department of Veterinary Public Health, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan

Correspondence
Wataru Yamazaki
wataru{at}iph.pref.osaka.jp

Received 11 October 2007
Accepted 4 January 2008

We developed a loop-mediated isothermal amplification (LAMP) assay for the rapid and simple detection of Campylobacter jejuni and Campylobacter coli. The assay provides a specific LAMP product for each of these two species. The assay correctly identified 65 C. jejuni and 45 C. coli strains, but not 75 non-C. jejuni/coli strains. The sensitivity of the LAMP assay for C. jejuni and C. coli in spiked human stool specimens was 5.6x103 c.f.u. g–1 (1.4 c.f.u. per test tube) and 4.8x103 c.f.u. g–1 (1.2 c.f.u. per test tube), respectively. When 90 stool specimens from patients with diarrhoea were tested by LAMP and direct plating, the LAMP results showed 81.3 % sensitivity and 96.6 % specificity compared to isolation of C. jejuni and C. coli by direct plating. Further, the LAMP assay required less than 2 h for detection of C. jejuni and C. coli in stool specimens. This LAMP assay is a rapid and simple tool for the detection of C. jejuni and C. coli and will be useful in facilitating the early diagnosis of food poisoning incidents caused by these organisms.

Abbreviations: LAMP, loop-mediated isothermal amplification.






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