J Med Microbiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collery, M. M.
Right arrow Articles by Smyth, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collery, M. M.
Right arrow Articles by Smyth, C. J.
Agricola
Right arrow Articles by Collery, M. M.
Right arrow Articles by Smyth, C. J.
J Med Microbiol 57 (2008), 348-358; DOI: 10.1099/jmm.0.47734-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Molecular typing of nasal carriage isolates of Staphylococcus aureus from an Irish university student population based on toxin gene PCR, agr locus types and multiple locus, variable number tandem repeat analysis

Mark M. Collery1, Davida S. Smyth1,{dagger}, Jane M. Twohig1, Anna C. Shore2, David C. Coleman2 and Cyril J. Smyth1

1 Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, University of Dublin, Dublin 2, Republic of Ireland

2 Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Dublin 2, Republic of Ireland

Correspondence
Cyril J. Smyth
csmyth{at}tcd.ie

Received 30 October 2007
Accepted 27 November 2007

Forty-eight Staphylococcus aureus isolates collected from a young, healthy, Irish university student population from 1995 to 2004 were screened for 16 enterotoxin (SE) and enterotoxin-like (SEl) genes (sea–see, seg–sei, selj–selo, selq, selu), and for the toxic shock toxin syndrome toxin-1 gene, tst. All of the isolates harboured at least one SE or SEl gene and 66.7 % possessed a classical SE gene (sea, seb, sec), the commonest being the seb gene. Most of the isolates (85.4 %) had a complete egc locus (selo, selm, sei, seln, seg). The intergenic sei–seln region of the egc locus was typed by PCR-RFLP in 34 isolates, 15 possessing pseudogenes {psi}ent1 and {psi}ent2 and 19 having the selu gene. The seh and sell genes, the selk–selq gene combination, and the tst gene were each found in <15 % of isolates. The agr genotype distribution was agr type III, 37.5 %; agr type I, 35.4 %; agr type II, 25 %; and agr type IV, 2.1 %. There was no association between SE–SEl genotype and agr type. All tst gene-positive isolates were of agr type III and harboured a classical SE gene. Multiple locus, variable number tandem repeat analysis (MLVA) produced 47 different patterns. While the sdr locus was present in all isolates, half of them lacked one or two of the sdr gene amplimers. Twenty isolates harboured the bbp gene, its presence being associated with agr type III, but not with the SE–SEl gene profile. The agr types of isolates were associated with MLVA subclusters. Selective MLST analysis revealed seven novel sequence types and a new aroE allele. Five clonal clusters (CCs), including CCs comprising major pandemic clones CC30, CC5 and CC22 and minor lineages CC6 and CC9, and three singletons were identified.

Abbreviations: CC, clonal cluster; MLVA, multiple locus, variable number tandem repeat analysis; PFGE, pulsed-field gel electrophoresis; SE, staphylococcal enterotoxin; SEl, staphylococcal enterotoxin-like; slv, single locus variant; ST, sequence type; TSST-1, toxic shock syndrome toxin-1.

{dagger}Present address: New York Medical College, Department of Microbiology and Immunology, Valhalla, NY 10595, USA.




This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
R. Ruimy, L. Armand-Lefevre, F. Barbier, E. Ruppe, R. Cocojaru, Y. Mesli, A. Maiga, M. Benkalfat, S. Benchouk, H. Hassaine, et al.
Comparisons between Geographically Diverse Samples of Carried Staphylococcus aureus
J. Bacteriol., September 15, 2009; 191(18): 5577 - 5583.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
M. M. Collery, D. S. Smyth, J. J. G. Tumilty, J. M. Twohig, and C. J. Smyth
Associations between enterotoxin gene cluster types egc1, egc2 and egc3, agr types, enterotoxin and enterotoxin-like gene profiles, and molecular typing characteristics of human nasal carriage and animal isolates of Staphylococcus aureus
J. Med. Microbiol., January 1, 2009; 58(1): 13 - 25.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. C. Shore, A. S. Rossney, B. O'Connell, C. M. Herra, D. J. Sullivan, H. Humphreys, and D. C. Coleman
Detection of Staphylococcal Cassette Chromosome mec-Associated DNA Segments in Multiresistant Methicillin-Susceptible Staphylococcus aureus (MSSA) and Identification of Staphylococcus epidermidis ccrAB4 in both Methicillin-Resistant S. aureus and MSSA
Antimicrob. Agents Chemother., December 1, 2008; 52(12): 4407 - 4419.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2008 Society for General Microbiology.