J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Sorhouet Pereira, C.
Right arrow Articles by Mollerach, M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sorhouet Pereira, C.
Right arrow Articles by Mollerach, M.
Agricola
Right arrow Articles by Sorhouet Pereira, C.
Right arrow Articles by Mollerach, M.
J Med Microbiol 57 (2008), 338-342; DOI: 10.1099/jmm.0.47631-0
© 2008 Society for General Microbiology
ISSN 1473-5644

PorA types in Neisseria meningitidis serogroup B isolated in Argentina from 2001 to 2003: implications for the design of an outer membrane protein-based vaccine

Cecilia Sorhouet Pereira1,2, Mabel Regueira2 and Marta Mollerach1

1 Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956, 1113 Buenos Aires, Argentina

2 Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas – ANLIS ‘Dr Carlos G. Malbrán’, Av. Velez Sarsfield 563, 1281 Buenos Aires, Argentina

Correspondence
Marta Mollerach
mmollera{at}ffyb.uba.ar

Received 13 September 2007
Accepted 6 November 2007

Identification of Neisseria meningitidis PorA types remains important, as the PorA protein is a major immunogenic component of several meningococcal vaccines under development. In this study, 191 N. meningitidis serogroup B isolates collected in Argentina through active laboratory-based surveillance from 2001 to 2003 were serosubtyped. Nucleotide sequences of the porA variable region 1 (VR1) and VR2 regions were determined in 52 non-serosubtypeable isolates. A substantial number of distinct VR types were identified, and a new VR2 variant from the P1.16 family was described. This is the first report describing PorA types in N. meningitidis serogroup B isolates in Argentina. Furthermore, the wide diversity of subtypes detected by serosubtyping and genosubtyping reveals the difficulty in designing a useful outer-membrane vaccine applicable in this country. A possible mechanism responsible for altered PorA expression was analysed in two PorA types.

Abbreviations: INEI, Instituto Nacional de Enfermedades Infecciosas; FSST, fully serosubtyped; MenB, Neisseria meningitidis serogroup B; NSST, non-serosubtypeable; OMP, outer-membrane protein; PSST, partially serosubtyped; VR, variable region.

The GenBank/EMBL/DDBJ accession numbers for the porA sequences of the two non-serosubtypeable Neisseria meningitidis serogroup B isolates are DQ411822 and DQ453920.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2008 Society for General Microbiology.