J Med Microbiol
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Varela, G.
Right arrow Articles by Schelotto, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Varela, G.
Right arrow Articles by Schelotto, F.
Agricola
Right arrow Articles by Varela, G.
Right arrow Articles by Schelotto, F.
J Med Microbiol 57 (2008), 1518-1522; DOI: 10.1099/jmm.0.2008/001917-0
© 2008 Society for General Microbiology
ISSN 0022-2615

Prevalence and dissemination of the Ser315Thr substitution within the KatG enzyme in isoniazid-resistant strains of Mycobacterium tuberculosis isolated in Uruguay

Gustavo Varela1, Sabina González1, Pilar Gadea1, Cecilia Coitinho2, Inés Mota1, Gladys González1, Fernando Goñi3,4, Carlos Rivas2 and Felipe Schelotto1

1 Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay

2 Laboratory Department, Honorary Anti-Tuberculosis Commission, 18 de Julio 2175, Montevideo, Uruguay

3 Department of Immunology, School of Chemistry, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay

4 Department of Neurology, New York University School of Medicine, New York, USA

Correspondence
Gustavo Varela
gvarela{at}higiene.edu.uy

Received March 12, 2008
Accepted August 20, 2008

The aim of this study was to determine the prevalence of Ser315Thr substitution in isoniazid (INH)-resistant strains of Mycobacterium tuberculosis in Uruguay. The katG gene of 62 INH-resistant strains was analysed by an RFLP-PCR assay. PCR products were digested with MspI to detect Ser315Thr and Arg463Leu substitutions. A total of 16 of the 62 (26 %) INH-resistant strains analysed had a Ser315Thr substitution. Only one INH-resistant strain had an Arg463Leu substitution and two strains had a deletion in katG. Of the 16 strains with Ser315Thr, 15 showed different profiles using a double-repetitive-element PCR assay, demonstrating that there was no local dissemination of any particular strain. These findings are in agreement with published data from regions where the prevalence of tuberculosis (TB) is intermediate and may be due in part to the success of the local TB control programme.

Abbreviations: DRE, double repetitive element; INH, isoniazid; RIF, rifampicin; TB, tuberculosis.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2008 Society for General Microbiology.