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Characteristics of a pandemic clone of O3 : K6 and O4 : K68 Vibrio parahaemolyticus isolated in Beira, Mozambique

M. Ansaruzzaman¹, Ashrafuzzaman Chowdhury¹, Nurul A. Bhuiyan¹, Marzia Sultana¹, Ashrafus Safa¹, Marcelino Lucas², Lorenz von Seidlein³, Avertino Barreto², Claire-Lise Chaignat⁴, David A. Sack^1,, John D. Clemens³, G. Balakrish Nair^1,, Seon Young Choi^3,5, Yoon Seong Jeon^3,5,6, Je Hee Lee^3,5, Hye Ri Lee³, Jongsik Chun^3,5,6 and Dong Wook Kim³

¹ ICDDR,B (International Centre for Diarrhoeal Disease Research, Bangladesh), Mohakhali, Dhaka 1212, Bangladesh

² Ministry of Health, Maputo, Mozambique

³ International Vaccine Institute, Seoul, Republic of Korea

⁴ World Health Organization, Geneva, Switzerland

⁵ School of Biological Sciences, Seoul National University, Seoul, Republic of Korea

⁶ Interdisciplinary Program of Bioinformatics, Seoul National University, Seoul, Republic of Korea

Correspondence
Dong Wook Kim
dwkim{at}ivi.int

Received June 18, 2008
Accepted August 22, 2008

The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized diarrhoea patients in Beira, Mozambique, were serotyped for O : K antigens and genotyped for toxR, tdh and trh genes. A group-specific PCR, a PCR that detects the presence of ORF8 of the filamentous phage f237, arbitrarily primed PCR, PFGE and multilocus sequence typing were performed to determine the pandemic status of the strains and their ancestry. All strains of serovars O3 : K6 (n=38) and O4 : K68 (n=4) were identified as a pandemic clonal group by these analyses. These strains are closely related to the pandemic reference strains of O3 : K6 and O4 : K68, which emerged in Asia in 1996 and were later found globally. The pandemic serotypes O3 : K6 and O4 : K68 including reference strains grouped into a single cluster indicating emergence from a common ancestor. The O3 : K58 (n=8), O4 : K13 (n=6), O3 : KUT (n=1) and O8 : K41 (n=1) strains showed unique characteristics different from the pandemic clone.

Abbreviations: AP-PCR, arbitrarily primed PCR; GS-PCR, group-specific PCR; MLST, multilocus sequence typing; ST, sequence type; UPGMA, unweighted-pair grouping with mathematical averaging.

Present address: Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

Present address: National Institute of Cholera and Enteric Diseases, Kolkata, India.

The GenBank/EMBL/DDBJ accession numbers for the new allele types identified in this study are EU770328–EU770349.