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J Med Microbiol 57 (2008), 1481-1490; DOI: 10.1099/jmm.0.2008/003665-0
© 2008 Society for General Microbiology
ISSN 0022-2615

Simultaneous identification of molecular and mating types within the Cryptococcus species complex by PCR-RFLP analysis

Xiaobo Feng1, Zhirong Yao1, Daming Ren2 and Wanqing Liao3

1 Medical Mycology Laboratory, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, PR China

2 State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai, PR China

3 Medical Mycology Laboratory, Shanghai Changzheng Hospital, Shanghai, PR China

Correspondence
Zhirong Yao
zryaosmu{at}sohu.com

Received May 21, 2008
Accepted July 29, 2008

The Cryptococcus species complex consists of two species, Cryptococcus neoformans and Cryptococcus gattii, which cause systemic infections in both immunocompromised and immunocompetent patients. Both species have a bipolar mating system, with mating type (MAT) {alpha} being predominant in clinical and environmental isolates. The strains of the Cryptococcus species complex have been divided into eight major molecular types, which show differences in epidemiology, biology and pathogenicity. In this study, two PCR-RFLP analyses, based on the CAP1 and GEF1 genes, which are both located at the MAT locus, were developed for simultaneous identification of the molecular and mating types of isolates of the Cryptococcus species complex. The molecular and mating types of all 144 cryptococcal isolates, including rare subtypes, were successfully determined by both PCR-RFLP approaches. Pattern analysis of the AD hybrids revealed that the serotype A MATa allele in strains of AaD{alpha} derived from genotype VNB, whereas the serotype A MAT{alpha} allele among strains of A{alpha}Da and A{alpha}D{alpha} derived from molecular type VNI.

The GenBank/EMBL/DDBJ accession numbers for the CAP1 and GEF1 sequences for the reference strains of WM626, CBS1930, WM161, B4546 and WM779 are EU571727–EU571731 and EU571732–EU571736, respectively.






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