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J Med Microbiol 57 (2008), 1277-1281; DOI: 10.1099/jmm.0.2008/001107-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Phenotypic evaluation of the effect of anaerobiosis on some virulence attributes of Candida albicans

Edvaldo Antonio Ribeiro Rosa1,2, Rodrigo Nunes Rached1, Sérgio Aparecido Ignácio1, Rosimeire Takaki Rosa1, Wander José da Silva2,3, Joyce Yick Yee Yau2 and Lakshman Perera Samaranayake2

1 Laboratory of Stomatology, Faculty of Dentistry, Biological and Health Sciences Centre, Pontifical Catholic University of Paraná, Rua Imaculada Conceição 1155, Curitiba PR 80215-901, Brazil

2 Oral Biosciences Unit, Faculty of Dentistry, Prince Philip Dental Hospital, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR

3 Department of Prosthetic Dentistry, Faculty of Dentistry of Piracicaba, State University of Campinas, Avenida Limeira 901, Piracicaba SP 13414-903, Brazil

Correspondence
Edvaldo Antonio Ribeiro Rosa
edvaldo.rosa{at}pucpr.br

Received 7 February 2008
Accepted 4 June 2008

The current assumption that Candida albicans is a facultatively anaerobic organism has been widely accepted since its recovery from anoxic sites became common. However, the link between anaerobiosis and virulence remains uncertain. This study investigated the differential cell-surface hydrophobicity (CSH) using a hydrocarbon/water partition technique and analysed the differential secretion rates of secretory aspartyl proteases (Saps), esterase, chondroitinase and haemolysins of C. albicans strains recovered from periodontal pockets and non-periodontium-related intra-oral sites. For the enzymic tests, all strains from both sets were grown under aerobic and anaerobic conditions and the harvested cells were inoculated onto suitable normal or pre-reduced culture media in the presence or absence of molecular oxygen, respectively. The results showed that no variations were perceptible for CSH and chondroitinase (P >0.05). The secretion rates of esterase and haemolysins strongly decreased in an anoxic environment (P <0.0001). However, a consistent increment (P <0.0001) in Sap secretion was detected when cultures were grown under anaerobic conditions. Based on these results, it is suggested that the oxygen concentration in the atmosphere surrounding cells exerts a variable influence on the virulence attributes of C. albicans.

Abbreviations: CSH, cell-surface hydrophobicity; Sap, secretory aspartyl protease.






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