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Department of Paediatrics, University of Florence and Anna Meyer Childrens Hospital, Viale Pieraccini 24, Florence I-50139, Italy
Correspondence
Chiara Azzari
chiara.azzari{at}unifi.it
Received 2 February 2008
Accepted 22 April 2008
0.41; McNemar P=0.000008). The incidence of IPD in children below 2 years of age was 11.5/100 000 and 51.8/100 000 by cultural and molecular methods, respectively. Pneumococcal serotyping by multiplex sequential PCR was obtained in 19/22 samples. Real-time PCR and multiplex sequential PCR can be used directly on biological samples, improving the ability to diagnose IPD. The incidence of IPD appears 5–10 times higher by PCR than by cultural methods.
Abbreviations: CSF, cerebrospinal fluid; IPD, invasive pneumococcal disease; RT, real-time.
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