J Med Microbiol 57 (2008), 21-27; DOI: 10.1099/jmm.0.47252-0
© 2008 Society for General Microbiology
ISSN 1473-5644
Community-wide transmission of a strain of Mycobacterium tuberculosis that causes reduced lung pathology in mice
Sally A. Cantrell1,
Lisa Pascopella2,
Jennifer Flood2,
Charles M. Crane3,
Lon V. Kendall4 and
Lee W. Riley1
1 Program in Infectious Diseases and Immunity, School of Public Health, University of California, Berkeley, CA, USA
2 Tuberculosis Control Branch, California Department of Public Health, Richmond, CA, USA
3 Contra Costa Health Services, Martinez, CA, USA
4 Comparative Pathology Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA
Correspondence
Lee W. Riley
lwriley{at}berkeley.edu
Received 24 February 2007
Accepted 8 October 2007
Since 1992, Mycobacterium tuberculosis strain PG004 has been responsible for a large outbreak of tuberculosis in one northern Californian community. There are no epidemiological or host factors to explain this outbreak. PG004 was therefore analysed for biological characteristics that might explain its widespread distribution. BABL/c mice were infected intravenously with PG004, non-PG004 M. tuberculosis strains CCC20 and CCC23 isolated from patients in the same community, and the laboratory strain H37Rv. The susceptibility of PG004 to reactive nitrogen intermediates (RNIs) was compared with that of H37Rv. Because of the reported association of phenolic glycolipid production with mouse virulence, a junction sequence in the polyketide synthase gene cluster (pks15/1) was compared among strains. It was found that the most virulent strain, based on mouse mortality, was not the outbreak strain PG004, but the non-outbreak strain CCC20. This strain had an intact pks15/1 sequence identical to that of another non-outbreak strain, CCC23, which caused death in only one out of ten mice in 300 days of follow-up. The outbreak strain PG004 had a frameshift mutation in the pks15/1 sequence identical to the sequence of H37Rv, and it was no more resistant to RNIs than H37Rv. The most distinguishing feature of PG004 was its failure to produce well-organized, coalescing granulomas in mouse lungs. The lack of organized granulomas and reduced pathology may prevent restriction of PG004 in the lungs and allow it to spread into alveolar air spaces and escape the host to transmit to others. Humans with reduced lung pathology may remain undiagnosed and untreated in the community longer than those with severe disease. The over-representation of an M. tuberculosis strain in a community, therefore, may be more associated with strains that cause reduced rather than severe lung pathology.
Abbreviations: ASN, acidified sodium nitrite; IL, interleukin; PGL, phenolic glycolipid; PGRS, polymorphic GC-rich tandem repeat sequence; p.i., post-infection; RNIs, reactive nitrogen intermediates; ROIs, reactive oxygen intermediates; Th, T helper.
Copyright © 2008 Society for General Microbiology.
