J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, H.
Right arrow Articles by Ye, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, H.
Right arrow Articles by Ye, L.
Agricola
Right arrow Articles by Han, H.
Right arrow Articles by Ye, L.
J Med Microbiol 56 (2007), 1219-1223; DOI: 10.1099/jmm.0.47005-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Utility of mycobacterial interspersed repetitive unit typing for differentiating Mycobacterium tuberculosis isolates in Wuhan, China

Hui Han1,5, Fang Wang2, Yong Xiao3, Yi Ren3, Yanjie Chao4, Aizhen Guo4 and Lingxiang Ye1

1 Department of Epidemiology, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, China

2 Department of Social Medicine, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, China

3 Wuhan Tuberculosis Prevention and Treatment Hospital, Wuhan 430030, China

4 National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China

5 National Center for Women and Children's Health, China CDC, Beijing 100013, China

Correspondence
Lingxiang Ye
yelx2004{at}163.com

Received 13 October 2006
Accepted 10 May 2007

Mycobacterial interspersed repetitive unit (MIRU) typing has been found to allow rapid, reliable, high-throughput genotyping of Mycobacterium tuberculosis, and may represent a feasible approach to study M. tuberculosis molecular epidemiology. To evaluate the use of MIRU typing in discriminating M. tuberculosis strains, isolates from 105 patients in Wuhan City, China, were genotyped by this method as compared to spoligotyping. MIRU typing identified 55 types that defined 21 clusters and 34 unique isolates. The discriminatory power was high [Hunter–Gaston discriminatory index (HGDI), 0.97]. Spoligotyping showed that 86 (81.9 %) of 105 isolates belonged to the Beijing family genotype. For Beijing family and non-Beijing strains, the discriminatory power of MIRU was high (HGDI, 0.95 and 0.98, respectively). Among the alleles of the MIRU loci for the Beijing family, only locus 26 was highly discriminative, but for non-Beijing strains, loci 10, 16 and 26 were highly discriminative. MIRU typing is a simple and fast method which may be used for preliminary screening of M. tuberculosis isolates in China.

Abbreviations: HGDI, Hunter–Gaston discriminatory index; MIRU, mycobacterial interspersed repetitive unit; TB, tuberculosis; VNTR, variable number tandem repeat; WTPTH, Wuhan Tuberculosis Prevention and Treatment Hospital.




This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
C. Allix-Beguec, D. Harmsen, T. Weniger, P. Supply, and S. Niemann
Evaluation and Strategy for Use of MIRU-VNTRplus, a Multifunctional Database for Online Analysis of Genotyping Data and Phylogenetic Identification of Mycobacterium tuberculosis Complex Isolates
J. Clin. Microbiol., August 1, 2008; 46(8): 2692 - 2699.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
C. Allix-Beguec, M. Fauville-Dufaux, and P. Supply
Three-Year Population-Based Evaluation of Standardized Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis
J. Clin. Microbiol., April 1, 2008; 46(4): 1398 - 1406.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.