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J Med Microbiol 56 (2007), 1219-1223; DOI: 10.1099/jmm.0.47005-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Utility of mycobacterial interspersed repetitive unit typing for differentiating Mycobacterium tuberculosis isolates in Wuhan, China

Hui Han1,5, Fang Wang2, Yong Xiao3, Yi Ren3, Yanjie Chao4, Aizhen Guo4 and Lingxiang Ye1

1 Department of Epidemiology, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, China

2 Department of Social Medicine, School of Public Health, Huazhong University of Science and Technology, Wuhan 430030, China

3 Wuhan Tuberculosis Prevention and Treatment Hospital, Wuhan 430030, China

4 National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China

5 National Center for Women and Children's Health, China CDC, Beijing 100013, China

Correspondence
Lingxiang Ye
yelx2004{at}163.com

Received 13 October 2006
Accepted 10 May 2007

Mycobacterial interspersed repetitive unit (MIRU) typing has been found to allow rapid, reliable, high-throughput genotyping of Mycobacterium tuberculosis, and may represent a feasible approach to study M. tuberculosis molecular epidemiology. To evaluate the use of MIRU typing in discriminating M. tuberculosis strains, isolates from 105 patients in Wuhan City, China, were genotyped by this method as compared to spoligotyping. MIRU typing identified 55 types that defined 21 clusters and 34 unique isolates. The discriminatory power was high [Hunter–Gaston discriminatory index (HGDI), 0.97]. Spoligotyping showed that 86 (81.9 %) of 105 isolates belonged to the Beijing family genotype. For Beijing family and non-Beijing strains, the discriminatory power of MIRU was high (HGDI, 0.95 and 0.98, respectively). Among the alleles of the MIRU loci for the Beijing family, only locus 26 was highly discriminative, but for non-Beijing strains, loci 10, 16 and 26 were highly discriminative. MIRU typing is a simple and fast method which may be used for preliminary screening of M. tuberculosis isolates in China.

Abbreviations: HGDI, Hunter–Gaston discriminatory index; MIRU, mycobacterial interspersed repetitive unit; TB, tuberculosis; VNTR, variable number tandem repeat; WTPTH, Wuhan Tuberculosis Prevention and Treatment Hospital.




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