J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dias, C. A.
Right arrow Articles by Beall, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dias, C. A.
Right arrow Articles by Beall, B.
Agricola
Right arrow Articles by Dias, C. A.
Right arrow Articles by Beall, B.
J Med Microbiol 56 (2007), 1185-1188; DOI: 10.1099/jmm.0.47347-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children

Cícero A. Dias1,2, Lúcia Martins Teixeira2, Maria da Glória Carvalho3 and Bernard Beall3

1 Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, and Hospital Mãe de Deus, Porto Alegre, Rio Grande do Sul, Brazil

2 Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil

3 Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA

Correspondence
Bernard Beall
BBeall{at}cdc.gov

Received 16 April 2007
Accepted 24 May 2007

Capsular serotype surveillance of clinical isolates of Streptococcus pneumoniae is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6 %) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.

Abbreviations: CDC, Centers for Disease Control and Prevention.




This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
F. C. Pimenta, R. E. Gertz Jr., A. Roundtree, J. Yu, M. H. Nahm, R. R. McDonald, M. da Gloria Carvalho, and B. W. Beall
Rarely Occurring 19A-Like cps Locus from a Serotype 19F Pneumococcal Isolate Indicates Continued Need of Serology-Based Quality Control for PCR-Based Serotype Determinations
J. Clin. Microbiol., July 1, 2009; 47(7): 2353 - 2354.
[Full Text] [PDF]

Home page
 J Med MicrobiolHome page
M. Antonio, I. Hakeem, K. Sankareh, Y. B. Cheung, and R. A. Adegbola
Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions
J. Med. Microbiol., March 1, 2009; 58(3): 296 - 302.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
L. Morais, M. d. G. Carvalho, A. Roca, B. Flannery, I. Mandomando, M. Soriano-Gabarro, B. Sigauque, P. Alonso, and B. Beall
Sequential multiplex PCR for identifying pneumococcal capsular serotypes from south-Saharan African clinical isolates
J. Med. Microbiol., September 1, 2007; 56(9): 1181 - 1184.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.