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J Med Microbiol 56 (2007), 1185-1188; DOI: 10.1099/jmm.0.47347-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children

Cícero A. Dias1,2, Lúcia Martins Teixeira2, Maria da Glória Carvalho3 and Bernard Beall3

1 Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, and Hospital Mãe de Deus, Porto Alegre, Rio Grande do Sul, Brazil

2 Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil

3 Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA

Correspondence
Bernard Beall
BBeall{at}cdc.gov

Received 16 April 2007
Accepted 24 May 2007

Capsular serotype surveillance of clinical isolates of Streptococcus pneumoniae is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6 %) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.

Abbreviations: CDC, Centers for Disease Control and Prevention.




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