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J Med Microbiol 56 (2007), 1181-1184; DOI: 10.1099/jmm.0.47346-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Sequential multiplex PCR for identifying pneumococcal capsular serotypes from south-Saharan African clinical isolates

Luis Morais1, Maria da Glória Carvalho2, Anna Roca1,3, Brendan Flannery2, Inacio Mandomando1,4, Montserrat Soriano-Gabarró2, Betuel Sigauque1,4, Pedro Alonso1,3 and Bernard Beall2

1 Centro de Investigação em Saúde da Manhiça (CISM), Ministerio de Saúde, CP1929 Maputo, Mozambique

2 Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA

3 Centre de Salut Internacional (CSI), Hospital Clinic/IDIBAPS, Universitat de Barcelona, Barcelona, Spain

4 Instituto Nacional de Saúde Ministério de Saúde, Maputo, Mozambique

Correspondence
Bernard Beall
BBeall{at}cdc.gov

Received 16 April 2007
Accepted 24 May 2007

A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8 %) of 153 isolates; 126 (82.4 %) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.

Abbreviations: CDC, Centers for Disease Control and Prevention.




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