J Med Microbiol
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morais, L.
Right arrow Articles by Beall, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morais, L.
Right arrow Articles by Beall, B.
Agricola
Right arrow Articles by Morais, L.
Right arrow Articles by Beall, B.
J Med Microbiol 56 (2007), 1181-1184; DOI: 10.1099/jmm.0.47346-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Sequential multiplex PCR for identifying pneumococcal capsular serotypes from south-Saharan African clinical isolates

Luis Morais1, Maria da Glória Carvalho2, Anna Roca1,3, Brendan Flannery2, Inacio Mandomando1,4, Montserrat Soriano-Gabarró2, Betuel Sigauque1,4, Pedro Alonso1,3 and Bernard Beall2

1 Centro de Investigação em Saúde da Manhiça (CISM), Ministerio de Saúde, CP1929 Maputo, Mozambique

2 Respiratory Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA

3 Centre de Salut Internacional (CSI), Hospital Clinic/IDIBAPS, Universitat de Barcelona, Barcelona, Spain

4 Instituto Nacional de Saúde Ministério de Saúde, Maputo, Mozambique

Correspondence
Bernard Beall
BBeall{at}cdc.gov

Received 16 April 2007
Accepted 24 May 2007

A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8 %) of 153 isolates; 126 (82.4 %) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.

Abbreviations: CDC, Centers for Disease Control and Prevention.




This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
B.-M. Njanpop Lafourcade, O. Sanou, M. van der Linden, N. Levina, M. Karanfil, S. Yaro, T. A. Tamekloe, and J. E. Mueller
Serotyping Pneumococcal Meningitis Cases in the African Meningitis Belt by Use of Multiplex PCR with Cerebrospinal Fluid
J. Clin. Microbiol., February 1, 2010; 48(2): 612 - 614.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
F. C. Pimenta, R. E. Gertz Jr., A. Roundtree, J. Yu, M. H. Nahm, R. R. McDonald, M. da Gloria Carvalho, and B. W. Beall
Rarely Occurring 19A-Like cps Locus from a Serotype 19F Pneumococcal Isolate Indicates Continued Need of Serology-Based Quality Control for PCR-Based Serotype Determinations
J. Clin. Microbiol., July 1, 2009; 47(7): 2353 - 2354.
[Full Text] [PDF]

Home page
 J Med MicrobiolHome page
M. Antonio, I. Hakeem, K. Sankareh, Y. B. Cheung, and R. A. Adegbola
Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions
J. Med. Microbiol., March 1, 2009; 58(3): 296 - 302.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
C. A. Dias, L. M. Teixeira, M. d. G. Carvalho, and B. Beall
Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children
J. Med. Microbiol., September 1, 2007; 56(9): 1185 - 1188.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.