J Med Microbiol 56 (2007), 1076-1080; DOI: 10.1099/jmm.0.47206-0
© 2007 Society for General Microbiology
ISSN 1473-5644
Dissemination of imipenem-resistant Acinetobacter baumannii strains carrying the ISAba1–blaOXA-23 genes in a Chinese hospital
Hua Zhou,
Bo-Rui Pi,
Qing Yang,
Yun-Song Yu,
Ya-Gang Chen,
Lan-Juan Li and
Shu-Sen Zheng
The Key Laboratory of Infectious Disease of the Public Health Ministry, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China
Correspondence
Yun-Song Yu
yvys119{at}163.com
Received 2 February 2007
Accepted 2 April 2007
An outbreak of 95 clinical infections with imipenem-resistant Acinetobacter baumannii in a Chinese hospital was investigated and the carbapenemase-encoding genes and their relationship with ISAba1 of these and a further 16 isolates recovered from the intensive care unit (ICU) environment were analysed. Almost all isolates were resistant to a wide range of antimicrobials; the lowest resistance rates were found for polymyxin E (17.1 %), cefoperazone/sulbactam (30.6 %) and ampicillin/sulbactam (67.6 %). Six pattern types defined by DNA macrorestriction patterns were distinguished among the clinical isolates with dissemination of pattern A (50 isolates) to patients in seven hospital units and pattern B (35 isolates) to eight units; the environmental isolates from ICUs were also of pattern A. All isolates were positive for the blaOXA-66 and blaOXA-23 genes. The OXA-23-encoding gene was located 34 bp downstream of ISAba1. No plasmids were detected and conjugal transfer of resistance was not demonstrated. The blaOXA-23 probe hybridized with 200 and 220 kb ApaI chromosomal fragments for type patterns A and B, respectively.
Abbreviations: ICU, intensive care unit.
The GenBank/EMBL/DDBJ accession number for the ISAba1–OXA-23 sequence reported in this work is DQ923478.
This article has been cited by other articles:

|
 |

|
 |
 
L. Poirel, S. Figueiredo, V. Cattoir, A. Carattoli, and P. Nordmann
Acinetobacter radioresistens as a Silent Source of Carbapenem Resistance for Acinetobacter spp.
Antimicrob. Agents Chemother.,
April 1, 2008;
52(4):
1252 - 1256.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Zhou, Q. Yang, Y.-S. Yu, Z.-Q. Wei, and L.-J. Li
Clonal Spread of Imipenem-Resistant Acinetobacter baumannii among Different Cities of China
J. Clin. Microbiol.,
December 1, 2007;
45(12):
4054 - 4057.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2007 Society for General Microbiology.