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J Med Microbiol 56 (2007), 918-920; DOI: 10.1099/jmm.0.46947-0
© 2007 Society for General Microbiology
ISSN 1473-5644

A specific real-time PCR assay for the detection of Bordetella pertussis

Benoit Vincart1, Ricardo De Mendonça1, Sylvianne Rottiers1, Françoise Vermeulen2, Marc J. Struelens1 and Olivier Denis1

1 Center for Molecular Diagnosis, Department of Microbiology, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium

2 Department of Paediatrics, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium

Correspondence
Benoit Vincart
bvincart{at}ulb.ac.be

Received 15 September 2006
Accepted 13 March 2007

A novel real-time PCR (RT-PCR) assay was developed for detection of Bordetella pertussis in respiratory specimens by targeting the pertactin gene. In vitro evaluation with reference strains and quality control samples showed analytical sensitivity equivalent to and specificity superior to those of PCR assays which target the IS481 element. The pertactin-based RT-PCR assay offers better discrimination between B. pertussis and other Bordetella species than previously described assays.

Abbreviations: RT-PCR, real-time PCR.




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B. Vincart, M. Hallin, M. J. Struelens, and O. Denis
Authors' reply to 'Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described RT-PCR targeting the pertactin gene'
J. Med. Microbiol., March 1, 2008; 57(3): 399 - 400.
[Full Text] [PDF]

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 J Med MicrobiolHome page
K. B. Register and T. L. Nicholson
Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described real-time PCR targeting the pertactin gene
J. Med. Microbiol., December 1, 2007; 56(12): 1608 - 1610.
[Abstract] [Full Text] [PDF]


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