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J Med Microbiol 56 (2007), 884-887; DOI: 10.1099/jmm.0.47127-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Identification of a gene encoding adaptin-like protein in the Paracoccidioides brasiliensis genome by random amplified polymorphic DNA analysis

Patrícia Ferrari Andreotti1, Juliana Leal Monteiro da Silva1, Elaine Cristina Teixeira2, Maria Célia Bertolini2, Christiane Pienna Soares1, Gil Benard3 and Maria José Soares Mendes-Giannini1

1 Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil

2 Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, Araraquara, SP, Brazil

3 Laboratório de Dermatologia e Imunodeficiências, Departamento de Dermatologia, Faculdade de Medicina, Universidade de São Paulo, Brazil

Correspondence
Maria José Soares Mendes-Giannini
giannini{at}fcfar.unesp.br

Received 18 December 2006
Accepted 8 March 2007

Paracoccidioides brasiliensis isolates are not homogeneous in their patterns of pathogenicity in animals and adhesion to epithelial cells. During this investigation, genotypic differences were observed between two samples of P. brasiliensis strain 18 yeast phase (Pb18) previously cultured many times, one taken before (Pb18a) and the other after (Pb18b) animal inoculation. Random amplified polymorphic DNA analysis using the primer OPJ4 distinguished Pb18b from Pb18a by one 308 bp DNA fragment, which after cloning and sequencing was shown to encode a polypeptide sequence homologous to the protein ß-adaptin. It is suggested, by comparison to other micro-organisms, that this protein might play an important role in the virulence of P. brasiliensis. This result demonstrates the influence of in vitro subculturing on the genotype of this organism.

Abbreviations: RAPD, random amplified polymorphic DNA.

The GenBank/EMBL/DDBJ accession no. for the adaptin-like protein sequence of Paracoccidioides brasiliensis is AY675222.






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