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J Med Microbiol 56 (2007), 803-808; DOI: 10.1099/jmm.0.46998-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Two diallyl sulphides derived from garlic inhibit meticillin-resistant Staphylococcus aureus infection in diabetic mice

Shih-Ming Tsao1, Wen-Hu Liu2 and Mei-Chin Yin2

1 Department of Infection, Chung Shan Medical University Hospital, Taichung City, Taiwan, Republic of China

2 Department of Nutritional Science, Chung Shan Medical University, Taichung City, Taiwan, Republic of China

Correspondence
Mei-Chin Yin
mcyin{at}csmu.edu.tw

Received 12 October 2006
Accepted 20 February 2007

The inhibitory effect of diallyl sulphide (DAS) and diallyl disulphide (DADS) against meticillin-resistant Staphylococcus aureus (MRSA) infection in diabetic mice was studied. The influence of these agents on the plasma levels of fibronectin, C-reactive protein (CRP), fibrinogen, interleukin (IL)-6 and tumour necrosis factor-{alpha} (TNF-{alpha}), and on the activity of plasminogen activator inhibitor-1 (PAI-1), antithrombin III (AT-III) and protein C, in MRSA-infected diabetic mice was examined. To induce diabetes, mice were treated intraperitoneally with streptozotocin for 5 consecutive days. Ten clinical MRSA isolates obtained from infected patients were used in this study. Diabetic mice were infected by injecting 200 µl MRSA/PBS suspension containing 107 c.f.u. via the tail vein. At day 4 post-infection, 200 µl DAS or DADS was administrated twice orally with an interval of 12 h. Eight hours after each administration, the blood and organs of mice were collected. Results showed that DAS and DADS significantly decreased MRSA viability in the kidney (P <0.05), with administration of each agent twice showing a greater inhibitory effect than when given once (P <0.05). MRSA infection in diabetic mice significantly elevated the plasma levels of IL-6 and TNF-{alpha} (P <0.05). DAS or DADS given once did not affect the plasma levels of IL-6 and TNF-{alpha} (P >0.05); however, DAS or DADS given twice significantly decreased the plasma levels of both IL-6 and TNF-{alpha} (P <0.05). DAS and DADS treatments also significantly reduced the plasma levels of CRP, fibronectin and fibrinogen (P <0.05). DAS or DADS treatment did not affect PAI-1 activity (P >0.05), but DAS or DADS given twice significantly increased AT-III activity (P <0.05). DADS given twice elevated protein C activity (P <0.05). MRSA infection significantly increased malondialdehyde levels in the kidney and spleen (P <0.05), and these levels were significantly decreased by treatment with DAS or DADS (P <0.05). These data suggest that DAS and DADS could provide multiple protective functions against MRSA infection in diabetic mice.

Abbreviations: AT-III, antithrombin III; CRP, C-reactive protein; DADS, diallyl disulphide; DAS, diallyl sulphide; IL-6, interleukin-6; MDA, malondialdehyde; MRSA, meticillin-resistant Staphylococcus aureus; PAI-1, plasminogen activator inhibitor-1; TNF-{alpha}, tumour necrosis factor-{alpha}.






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