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J Med Microbiol 56 (2007), 762-765; DOI: 10.1099/jmm.0.46625-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Evaluation of Albicans ID2 and Biggy agar for the isolation and direct identification of vaginal yeast isolates

Macit Ilkit1, Suleyha Hilmioglu2, Meltem Tasbakan2 and Sohret Aydemir2

1 Department of Microbiology, Faculty of Medicine, University of Çukurova, Adana, Turkey

2 Department of Microbiology, Faculty of Medicine, University of Ege, Izmir, Turkey

Correspondence
Macit Ilkit
milkit{at}cu.edu.tr

Received 12 March 2006
Accepted 8 February 2007

In this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, Albicans ID2 and Biggy agar, as well as onto Sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. These strains were identified as Candida glabrata in 20 (31.8 %) samples, Candida albicans in 15 samples (23.8 %), Candida tropicalis in 10 samples (15.9 %), Candida krusei in five samples (7.9 %), Candida kefyr in five samples (7.9 %), Candida dubliniensis in four samples (6.3 %), Candida parapsilosis in two samples (3.2 %) and Candida guilliermondii in two samples (3.2 %). Mixed fungal cultures and bacterial growth or filamentous fungi were not detected on any of the selected media. The sensitivity and specificity of the Albicans ID2 and Biggy agar with regard to the identification of C. albicans were 80.0 and 64.6 %, and 86.7 and 56.3 %, respectively. This study showed these two chromogenic media to be as effective as Sabouraud chloramphenicol agar with respect to fungal detection. However, neither Albicans ID2 nor Biggy agar was sufficient for reliable differentiation of yeasts to the species level.






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