J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Tables
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Malke, H.
Right arrow Articles by Ferretti, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Malke, H.
Right arrow Articles by Ferretti, J. J.
Agricola
Right arrow Articles by Malke, H.
Right arrow Articles by Ferretti, J. J.
J Med Microbiol 56 (2007), 707-714; DOI: 10.1099/jmm.0.46984-0
© 2007 Society for General Microbiology
ISSN 1473-5644

CodY-affected transcriptional gene expression of Streptococcus pyogenes during growth in human blood

Horst Malke and Joseph J. Ferretti

Oklahoma University Health Sciences Center, Department of Microbiology and Immunology, Oklahoma City, OK 73190, USA

Correspondence
Horst Malke
horst-malke{at}ouhsc.edu

Received 4 October 2006
Accepted 27 January 2007

In an attempt to expand the available knowledge of pathogen–host interactions during ex vivo growth of Streptococcus pyogenes (GAS) in nonimmune whole human blood, the extents to which the expression of 51 genes including regulators with known targets, established virulence factors, physiologically important transporters and metabolic enzyme genes was differentially affected in the presence or absence of a functional codY gene were determined. The results obtained by quantitative real-time PCR using the M49 strain NZ131 showed that CodY influenced GAS gene activity in a dynamic fashion, with differential responses detected for 26 genes and occasionally characterized by discordance in the blood environment compared to laboratory medium. Degenerate derivatives of the recently discovered CodY box potentially serving as a cis-regulatory element for CodY action were identified in the upstream regions of 15 genes of the NZ131 genome, and these genes featured sequence motifs identical to the NZ131 CodY box in all completely sequenced S. pyogenes genomes. As none of these genes represented a genuine virulence factor, it seems likely, therefore, that the observed differential transcription of the majority of virulence genes was caused by indirect actions of CodY as part of a regulatory network.

Abbreviations: GAS, group A streptococcus.

Tables showing the targets and primers used for real-time reverse transcription PCR and the mean transcript values of the genes and strains are available as supplementary material with the online version of this paper.




This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
G. A. Somerville and R. A. Proctor
At the Crossroads of Bacterial Metabolism and Virulence Factor Synthesis in Staphylococci
Microbiol. Mol. Biol. Rev., June 1, 2009; 73(2): 233 - 248.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Pohl, P. Francois, L. Stenz, F. Schlink, T. Geiger, S. Herbert, C. Goerke, J. Schrenzel, and C. Wolz
CodY in Staphylococcus aureus: a Regulatory Link between Metabolism and Virulence Gene Expression
J. Bacteriol., May 1, 2009; 191(9): 2953 - 2963.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. D. Majerczyk, M. R. Sadykov, T. T. Luong, C. Lee, G. A. Somerville, and A. L. Sonenshein
Staphylococcus aureus CodY Negatively Regulates Virulence Gene Expression
J. Bacteriol., April 1, 2008; 190(7): 2257 - 2265.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. R. Belitsky and A. L. Sonenshein
Genetic and Biochemical Analysis of CodY-Binding Sites in Bacillus subtilis
J. Bacteriol., February 15, 2008; 190(4): 1224 - 1236.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.