J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
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J Med Microbiol 56 (2007), 675-680; DOI: 10.1099/jmm.0.47020-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Enhancement of salivary IgA response to a DNA vaccine against Streptococcus mutans wall-associated protein A in mice by plasmid-based adjuvants

Thomas K. Han{dagger} and My Lien Dao

Department of Biology, University of South Florida, Tampa, FL, USA

Correspondence
My Lien Dao
mdao{at}cas.usf.edu

Received 22 October 2006
Accepted 8 January 2007

A specific salivary IgA (sIgA) response was obtained in mice by intranasal immunization with a naked DNA vaccine consisting of the Streptococcus mutans wall-associated protein A gene (wapA) inserted into the mammalian expression vector pcDNA3.1/V5/His-TOPO. In the present study, the vaccine, referred to as pcDNA-wapA, was administered with or without the cationic lipid DMRIE-C. No mucosal response was observed in mice immunized with the vaccine alone, whereas a weak and temporal sIgA response was obtained when the vaccine was mixed with DMRIE-C. To investigate the use of pcDNA containing the interleukin 5 (IL-5) gene (pcDNA-il-5) or the cholera toxin B gene (pcDNA-ctb) as genetic adjuvants, these constructs were used in co-immunization studies. The enhancement effect was transient with pcDNA-il-5, but longer lasting with pcDNA-ctb, thus supporting the use of the latter as a genetic adjuvant to DNA vaccine.

Abbreviations: GST, glutathione S-transferase; HRP, horseradish peroxidase; IL, interleukin; sIgA, salivary IgA.

{dagger}Present address: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, and Department of Medicine, Harvard Medical School, Boston MA, USA.






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