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J Med Microbiol 56 (2007), 524-532; DOI: 10.1099/jmm.0.46635-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Detection of a neonatal human rotavirus strain with VP4 and NSP4 genes of porcine origin

Joana D'Arc P. Mascarenhas1, José Paulo G. Leite2, Jackson C. Lima1, Marcos B. Heinemann2, Darleise S. Oliveira1, Irene T. Araújo2, Luana S. Soares1, Rosa Helena P. Gusmão3, Yvone B. Gabbay1 and Alexandre C. Linhares1

1 Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Belém, Brazil

2 Departamento de Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

3 Universidade do Estado do Pará, Belém, Brazil

Correspondence
Joana D'Arc P. Mascarenhas
joanamascarenhas{at}iec.pa.gov.br

Received 17 March 2006
Accepted 14 November 2006

A human rotavirus strain (NB-150) was detected in stool samples from a neonate hospitalized for mild/moderate community-acquired diarrhoea. This baby lived in the outskirts of Belém, Brazil, under poor sanitation conditions. The NB-150 strain displayed a typical long electrophoretic pattern with 11 gene segments. It had two VP7 alleles, G1 and G4, and belonged to VP6 subgroup II. A close relatedness with human rotaviruses was shown for VP7 alleles: G1 (96.9–100 % similarity at the amino acid level) and G4 (97.1–100 % similarity at the amino acid level). As for VP6, 95.1–97.5 % similarity at the amino acid level was noted. VP8* and NSP4 genes showed a close relatedness with those of porcine rotavirus strains, as follows: VP8* (95.0 % similarity at the amino acid level) and NSP4 (93.7–96.0 % similarity at the amino acid level). This is believed to be the first report in Brazil of a rotavirus infection involving a strain with G1 and G4 alleles, with VP8* and NSP4 genes of porcine origin. These findings strongly suggest the occurrence of interspecies transmission.

Abbreviations: EIA, enzyme immunoassay; e-type, RNA electropherotype; SG, subgroup.

The GenBank/EMBL/DDBJ accession numbers for the partial sequences of the neonatal rotavirus isolates are DQ299878 (VP6), DQ299877 (VP4), DQ299879 (VP7 G1), DQ299880 (VP7 G4) and DQ299876 (NSP4).




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