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J Med Microbiol 56 (2007), 514-518; DOI: 10.1099/jmm.0.46542-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Evaluation of susceptibility of Trichophyton mentagrophytes and Trichophyton rubrum clinical isolates to antifungal drugs using a modified CLSI microdilution method (M38-A)

Maria Elisabete da Silva Barros, Daniel de Assis Santos and Júnia Soares Hamdan

Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

Correspondence
Maria Elisabete da Silva Barros
betebarros{at}ef.ufop.br

Received 25 January 2006
Accepted 12 December 2006

Onychomycosis is a common adult human mycosis, and dermatophytes of the Trichophyton genera are the most common causative agent. Many antimycotic agents are safe and highly effective for the treatment of dermatophytosis, and are available for clinical practice. Successful treatment depends on the ability of antifungal drugs to eradicate the fungal isolates. The aim of this work was to determine the MICs of four antifungal drugs (fluconazole, itraconazole, terbinafine and griseofulvin) recognized for ungual dermatophytosis treatment caused by Trichophyton species, especially Trichophyton mentagrophytes and Trichophyton rubrum. MICs were determined using a broth microdilution method in accordance with Clinical and Laboratory Standards Institute approved standard M38-A with some modifications, such as an incubation temperature of 28 °C, an incubation time of 7 days and inocula constituted of only microconidia. The results showed that the activities of terbinafine and itraconazole were significantly higher (MICs of <0.007–0.031 and 0.015–0.25 µg ml–1, respectively) than other tested agents. All isolates had reduced susceptibility to fluconazole (1–64 µg ml–1). The MIC of griseofulvin varied among strains (MICs of 0.062–1 µg ml–1). The parameters adopted to perform susceptibility testing of T. rubrum and T. mentagrophytes to antifungal agents appeared to be suitable and reliable, and could contribute to the possible development of a standard protocol.

Abbreviations: CLSI, Clinical and Laboratory Standards Institute.






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