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J Med Microbiol 56 (2007), 495-499; DOI: 10.1099/jmm.0.46715-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Prospective evaluation of the new chromogenic medium CandiSelect 4 for differentiation and presumptive identification of the major pathogenic Candida species

Boualem Sendid1,2, Nadine François2, Annie Standaert1, Eric Dehecq3, Farid Zerimech4, Daniel Camus2 and Daniel Poulain1,2

1 Laboratoire de Mycologie Fondamentale & Appliquée; Inserm, U799; CHRU, Avenue J. Leclercq, 59045 Lille Cedex, France

2 Laboratoire de Parasitologie-Mycologie, CHRU, Avenue J. Leclercq, 59045 Lille Cedex, France

3 Laboratoire de Microbiologie, Hôpital Saint-Philibert, 115, rue du Grand-But, 59462 Lomme Cedex, France

4 Laboratoire de Biochimie, CHRU, Avenue J. Leclercq, 59045 Lille Cedex, France

Correspondence
Boualem Sendid
bsendid{at}univ-lille2.fr

Received 11 May 2006
Accepted 12 December 2006

The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the major pathogenic Candida species. The performance of CS4 was compared with that of another chromogenic medium, CHROMagar Candida (CA; Becton Dickinson). For primary cultures, 502 of the 1549 (32 %) samples were culture-positive. A total of 542 yeasts were isolated including 465 monomicrobial and 37 mixed cultures: 392 C. albicans, 60 Candida glabrata, 25 Candida tropicalis, 12 Candida krusei and 53 other Candida species. The percentage of C. albicans isolates that could be identified directly after 24, 48 and 72 h culture was 31.6, 82.9 and 92.1 %, respectively, for CS4, and 32.9, 82.9 and 91.1 % for CA. The presumptive identification of C. glabrata, C. tropicalis and C. krusei was evaluated after 48 h incubation. The percentage of strains with morphologically typical colonies was 80, 68 and 84.6 %, respectively, for CS4 compared with 75, 76 and 76.9 % for CA. For pure subcultures, from 24 h, all isolates of C. albicans (n=21) were directly identifiable on the two chromogenic media CA and CS4. At 48 h, the proportion of typical strains observed on the two chromogenic media was identical for C. glabrata (85 %) and C. krusei (100 %). A slight difference in favour of CS4 was observed for C. tropicalis (100 vs 95 %). CS4 also allowed the growth of several other fungi. CS4 can be recommended as a primary isolation medium for the identification of C. albicans, and for the rapid and effective differentiation of the major pathogenic Candida species.

Abbreviations: CA, CHROMagar Candida; CS4, CandiSelect 4.






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