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J Med Microbiol 56 (2007), 386-390; DOI: 10.1099/jmm.0.46608-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Molecular characterization of Clostridium difficile clinical isolates in a geriatric hospital

Isabelle Poilane1, Christel Humeniuk-Ainouz1, Isabelle Durand1, Claire Janoir2, Philippe Cruaud1, Michel Delmée3, Michel R. Popoff4 and Anne Collignon1,2

1 AP-HP, Hôpital Jean Verdier-René Muret, Service de Microbiologie, Bondy, France

2 Université de Paris-Sud-XI, Faculté de Pharmacie, USC INRA EA3534, Châtenay-Malabry, France

3 Université Catholique de Louvain, Unité de Microbiologie, Brussels, Belgium

4 Institut Pasteur, Unité des Bactéries Anaérobies et du Botulisme, Paris, France

Correspondence
Anne Collignon
anne.collignon{at}jvr.ap-hop-paris.fr

Received 4 March 2006
Accepted 8 September 2006

The discriminatory potential of a combination of various typing methods was evaluated on a set of 21 Clostridium difficile isolates obtained from symptomatic patients hospitalized in a geriatric unit and 7 non-toxigenic isolates from the same hospital. Isolates were firstly serotyped and toxinotyped. Of the 28 isolates, 19 belonged to serogroup A. PCR-ribotyping and PCR-RFLP on the fliC and slpA genes were then applied to these 19 isolates. The results suggest that the combination of PCR-ribotyping with PCR-RFLP analysis of slpA could be more discriminatory and suitable for studying C. difficile epidemiology.






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