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J Med Microbiol 56 (2007), 336-341; DOI: 10.1099/jmm.0.46738-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Molecular typing of a Legionella pneumophila outbreak in Ontario, Canada

Matthew W. Gilmour1,2, Kathryn Bernard1,2, Dobryan M. Tracz1, Adam B. Olson1, Cindi R. Corbett1, Tamara Burdz1, Betty Ng1, Deborah Wiebe1, George Broukhanski3, Peter Boleszczuk3, Patrick Tang3, Frances Jamieson3, Gary Van Domselaar1, Francis A. Plummer1,2 and Jody D. Berry1

1 National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada

2 Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada

3 Central Public Health Laboratory, Ministry of Health and Long Term Care, Toronto, Ontario, Canada

Correspondence
Matthew W. Gilmour
Matthew_Gilmour{at}phac-aspc.gc.ca

Received 23 May 2006
Accepted 10 November 2006

An outbreak of Legionnaires' disease at a long-term care facility in Ontario, Canada from September to October 2005 resulted in the death of 23 residents and the illness of 112 other people. In response, molecular methods were developed to detect Legionella pneumophila in clinical lung samples and to subtype isolates from clinical and environmental samples. The targeted genetic loci included Legionella-specific virulence determinants (mip, icmO, sidA and lidA) and core bacterial determinants (ftsZ, trpS and dnaX). An established amplified fragment length polymorphism typing method provided the first indication of genetic relatedness between strains recovered from clinical samples and strains cultured from environmental samples taken from the outbreak site. These associations were verified using the European Working Group for Legionella Infections sequence-based typing protocol targeting the flaA, pilE, asd, mip, mompS and proA loci. These molecular typing methods confirmed the outbreak source as a contaminated air conditioning cooling tower.

Abbreviations: AFLP, amplified fragment length polymorphism; DFA, direct fluorescent antibody assay; EWGLI, European Working Group for Legionella Infections; SBT, sequence-based typing.

The GenBank/EMBL/DDBJ accession numbers for the sequences of the new isolates are DQ451531–DQ451547.




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