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J Med Microbiol 56 (2007), 313-319; DOI: 10.1099/jmm.0.46685-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Adenoviruses associated with acute gastroenteritis in hospitalized and community children up to 5 years old in Rio de Janeiro and Salvador, Brazil

Edson Pereira Filho1,2, Nieli R. da Costa Faria2, Alexandre M. Fialho2, Rosane S. de Assis2, Marilda Maria S. Almeida2, Myrna Rocha3, Márcia Galvão4, Flávia B. dos Santos2, Maurício L. Barreto5 and José Paulo G. Leite2

1 Subdivisão de Pesquisa, Instituto de Biologia do Exército, Rua Francisco Manuel 102, Benfica, Rio de Janeiro, RJ, CEP 20911-270, Brazil

2 Departamento de Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Avenida Brasil 4365 – Pavilhão Cardoso Fontes, Manguinhos, Rio de Janeiro, RJ, CEP 21045-900, Brazil

3 Setor de Pediatria, Hospital Municipal Jesus, Rua Oito de Dezembro 717, Vila Isabel, Rio de Janeiro, RJ, CEP 20550-200, Brazil

4 Setor de Pediatria, Hospital Municipal Salles Neto, Praça Condessa Paulo de Frontin 52, Rio Comprido, Rio de Janeiro, RJ, CEP 20260-010, Brazil

5 Instituto de Saúde Coletiva, Universidade Federal da Bahia, Rua Padre Feijó 29, Canela, Salvador, BA, CEP 40110-170, Brazil

Correspondence
José Paulo G. Leite
jpgleite{at}ioc.fiocruz.br

Received 19 April 2006
Accepted 9 October 2006

Acute gastroenteritis is a major source of morbidity and mortality among young children in developed and developing countries. Human adenoviruses (HAdVs), and in particular species F, are related to childhood diarrhoea worldwide. This study presents the results obtained during an investigation of HAdVs causing acute gastroenteritis in children hospitalized in Rio de Janeiro, RJ, Brazil, from April 1996 to September 2003, as well as in children with diarrhoea living in the slums of Salvador, BA, Brazil, from October 2001 to September 2003. A total of 3060 stool samples was analysed by an enzyme immunoassay for rotavirus and adenovirus (EIARA) and 61 (2 %) were found to be positive. HAdV presented with low prevalence throughout the year, with a slight but not significant increase in incidence in late summer and early autumn. Children up to 2 years of age were the most frequently affected (79 % of all positive samples). All positive samples were analysed further by generic and species-specific HAdV PCR protocols, confirming 100 % specificity of this rapid and inexpensive EIARA. Species F was the most prevalent (65 %), despite the occurrence of species A (12 %), C, D and co-infection F/D (5 % each) and species B and co-infections F/A, F/C and B/D (2 % each). In order to type the species F strains as HAdV-40 or -41, generic PCR and a HinfI restriction digest were performed. HAdV-40 and -41 were found to represent 62 % (23/37) and 38 % (14/37), respectively. These results demonstrated that a combination of generic and species-specific PCRs is useful and reliable for HAdV species and type identification directly from faecal specimens. The results confirmed the endemism of human adenoviruses, mainly species F, in children as aetiological agents of diarrhoea, although the limited sensitivity of EIARA as a screening method may have underestimated their prevalence.

Abbreviations: EIARA, enzyme immunoassay for rotavirus and adenovirus; HAdV, human adenovirus.

The GenBank/EMBL/DDBJ accession numbers for the hexon gene sequences of the HAdV-41 samples determined in this work are DQ504432–DQ504434.






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