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J Med Microbiol 56 (2007), 298-304; DOI: 10.1099/jmm.0.46808-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Differentially expressed proteins of pathogenic Penicillium marneffei in yeast and mycelial phases

Liyan Xi1, Xiaorong Xu1, Wei Liu2, Xiqing Li1, Yulin Liu2, Mingtao Li2, Junmin Zhang1 and Mengfeng Li3

1 Department of Dermatology, Second Affiliated Hospital, Sun Yat-Sen University, 107 West Yanjiang Road, Guangzhou 510120, China

2 Center for Proteomics, School for Basic Medical Science, Sun Yat-Sen University, Guangzhou, China

3 Department of Microbiology, School for Basic Medical Science, Sun Yat-Sen University, Guangzhou, China

Correspondence
Liyan Xi
xiliyan{at}mail.sysu.edu.cn

Received 30 June 2006
Accepted 12 November 2006

Penicillium marneffei is a dimorphic fungus endemic in southeast Asia. The incidence of P. marneffei infection has increased greatly in this region with the spread of human immunodeficiency virus, but the infection routes and pathogenic mechanisms of P. marneffei remain poorly understood. P. marneffei is an opportunistic human pathogen exhibiting a temperature-dependent dimorphic switch. At 25 °C it grows as filamentous hyphae, whilst at 37 °C it forms uninucleate yeast cells and divides by fission. Dimorphic fungal pathogenicity is frequently associated with the dimorphic switch, but the mechanism that regulates the switch has remained obscure. In this report, two-dimensional difference gel electrophoresis was used to investigate the proteins expressed differentially in the yeast and mycelial phases of a wild-type isolate of P. marneffei. Among thousands of protein molecules displayed, more than 500 showed differential expression between the two phases. In particular, 26 proteins were identified using matrix-assisted laser desorption/ionization time-of-flight MS. Expression of catalase-peroxidase, isocitrate lyase, Hsp90, binding protein and cytochrome P-450 increased significantly in the yeast phase, whereas levels of poly(A) polymerase and SNF22 were reduced.

Abbreviations: 2D, two dimensional; 2D DIGE, two-dimensional difference gel electrophoresis; Hsp, heat-shock protein; MALDI-TOF MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.




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