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J Med Microbiol 56 (2007), 266-270; DOI: 10.1099/jmm.0.46760-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Haemoperfused liver as an ex vivo model for organ invasion of Candida albicans

Sascha Thewes1, Hilde-Kristin Reed2, Christian Grosse-Siestrup2, David A. Groneberg2, Michael Meissler2, Martin Schaller3 and Bernhard Hube1,{dagger}

1 Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany

2 Department of Comparative Medicine and Experimental Animal Science, Charité School of Medicine, 13353 Berlin, Germany

3 Institute of Dermatology, Eberhard Karls University, 72076 Tübingen, Germany

Correspondence
Bernhard Hube
bernard.hube{at}hki-jena.de

Received 2 June 2006
Accepted 13 October 2006

To study invasion of the human fungal pathogen Candida albicans, several infection models have been established. This study describes the successful establishment of an ex vivo haemoperfused liver as a model to study invasion of C. albicans. Perfused organs from pigs could be kept functional for up to 12 h. By comparing a non-invasive and invasive strain of C. albicans and by following a time course of invasion, it was shown that the invasion process in the perfused liver infection model is very similar to the in vivo situation after intraperitoneal infection of mice. The advantage of this set-up compared with other models of invasion is discussed.

Abbreviations: ALT, alanine aminotransferase; AST, aspartate aminotransferase; i.p., intraperitoneal; p.i., post-infection.

{dagger}Present address: Chair and Professor of Microbial Pathogenicity, Friedrich Schiller University Jena, Head of Department of Microbial Pathogenicity, Leibniz Institute for Natural Product Research and Infection Biology – Hans Knoell Institute Jena (HKI), Beutenbergstraß14;e 11a, 07745 Jena, Germany.




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