J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ansaruzzaman, M.
Right arrow Articles by Qadri, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ansaruzzaman, M.
Right arrow Articles by Qadri, F.
Agricola
Right arrow Articles by Ansaruzzaman, M.
Right arrow Articles by Qadri, F.
J Med Microbiol 56 (2007), 217-222; DOI: 10.1099/jmm.0.46473-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Characterization of enterotoxigenic Escherichia coli from diarrhoeal patients in Bangladesh using phenotyping and genetic profiling

M. Ansaruzzaman1, N. A. Bhuiyan1, Y. A. Begum1, I. Kühn2, G. B. Nair1, D. A. Sack1, A.-M. Svennerholm3 and F. Qadri1

1 ICDDR, B (International Centre for Diarrhoeal Diseases Research, Bangladesh), Centre for Health and Population Research, GPO Box 128, Dhaka-1000, Bangladesh

2 Microbiology and Tumorbiology Centre, Karolinska Institute, Stockholm, Sweden

3 Department of Medical Microbiology and Immunology, Goteborg University, S-413 46 Goteborg, Sweden

Correspondence
M. Ansaruzzaman
ansar{at}icddrb.org

Received 14 December 2005
Accepted 24 October 2006

A total of 99 isolates out of 370 colonization factor (CF)-positive, well-characterized enterotoxigenic Escherichia coli (ETEC) strains belonging to 13 different CF types isolated from diarrhoeal patients admitted to the hospital of the International Centre for Diarrhoeal Disease Research, Bangladesh, were tested. The isolates were selected at random based on expression of the major CFs prevailing in Dhaka, Bangladesh, from 1996 to 1998. These isolates were characterized by O-antigenic serotyping, randomly amplified polymorphic DNA (RAPD) analysis and biochemical fingerprinting using the PhenePlate (PhP) system. The 99 ETEC isolates belonged to 10 O serogroups, the predominant ones being O6 (n=28), O115 (n=20) and O128 (n=20). Most isolates of serogroup O6 (CS1+CS3, 11/14; CS2+CS3, 5/8) belonged to the same PhP/RAPD type (H/f), whereas other isolates of serogroup O6 (n=12) belonged to different PhP/RAPD types (Si/f and F/c). Eleven serogroup O128 (CFA/I) isolates belonged to the same PhP/RAPD type (E/b), whereas the other O128 isolates formed different PhP/RAPD types. Fifteen (75 %) serogroup O115 isolates (together with fourteen isolates from serogroups O25, O114, O142 and O159) demonstrated two closely related common groups by PhP typing (A and A1) and belonged to the same PhP/RAPD type (A/a). Three major clonal groups were identified among the ETEC strains in this study, largely based on O-antigenic type, CF expression pattern and toxin profile.

Abbreviations: CF, colonization factor; CFA, colonization factor antigen; ETEC, enterotoxigenic Escherichia coli; LT, heat-labile toxin; RAPD, random amplification of polymorphic DNA; ST, heat-stable toxin.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.