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J Med Microbiol 56 (2007), 190-195; DOI: 10.1099/jmm.0.46838-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Occurrence and genetic variability of Candida parapsilosis sensu lato in Hungary

Sándor Kocsubé1, Mónika Tóth1, Csaba Vágvölgyi1, Ilona Dóczi2, Miklós Pesti3, István Pócsi4, Judit Szabó5 and János Varga1,6

1 Department of Microbiology, Faculty of Sciences, University of Szeged, PO Box 533, H-6701 Szeged, Hungary

2 Department of Clinical Microbiology, Faculty of Medicine, University of Szeged, PO Box 427, H-6701 Szeged, Hungary

3 Department of General and Environmental Microbiology, University of Pécs, H-7601 Pécs, Hungary

4 Department of Microbiology and Biotechnology, Medical and Health Science Center, University of Debrecen, PO Box 63, H-4010, Debrecen, Hungary

5 Institute of Medical Microbiology, Faculty of Sciences, University of Debrecen, PO Box 63, H-4010, Debrecen, Hungary

6 CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands

Correspondence
János Varga
jvarga{at}cbs.knaw.nl

Received 17 July 2006
Accepted 13 October 2006

The occurrence and genetic variability of Candida parapsilosis isolates in two Hungarian hospitals, located in Debrecen and Pécs, were examined. Among the 209 Candida isolates examined, 20 were found to belong to C. parapsilosis sensu lato, based on morphological, physiological and molecular data. The frequency of occurrence of C. parapsilosis isolates (9.6 %) was lower than that observed in Europe but higher than that observed previously in Hungary. The genetic variability of C. parapsilosis sensu lato isolates was also examined using random amplified polymorphic DNA (RAPD) analysis and sequence analysis of the intergenic transcribed spacer (ITS) region of the rRNA gene cluster. The genetic variability of the isolates was relatively high, as revealed by RAPD analysis. Two isolates were found to belong to the recently described Candida metapsilosis species (C. parapsilosis group III), based on ITS sequence data, RAPD analysis and phenotypic data. These two isolates could also be distinguished from C. parapsilosis sensu stricto isolates using a primer pair developed for the detection of C. parapsilosis group I isolates. To the best of the authors' knowledge, this is the first report on the identification of C. metapsilosis from bloodstream infection.

Abbreviations: ITS, intergenic transcribed spacer; RAPD, random amplified polymorphic DNA.

The GenBank/EMBL/DDBJ accession numbers for the ITS sequences of C. metapsilosis 12821 and Bp57 are DQ786952 and DQ786953, respectively.

Neighbour-joining tree-based ITS sequence data of the examined Candida isolates are available as supplementary data with the online version of this paper.




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