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J Med Microbiol 56 (2007), 185-189; DOI: 10.1099/jmm.0.46790-0
© 2007 Society for General Microbiology
ISSN 1473-5644

PCR-based identification and strain typing of Pichia anomala using the ribosomal intergenic spacer region IGS1

Sonia Bhardwaj1, Rajeshwari Sutar2, Anand K. Bachhawat2, Sunit Singhi3 and Arunaloke Chakrabarti1

1 Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India

2 Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India

3 Department of Paediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India

Correspondence
Arunaloke Chakrabarti
arunaloke{at}hotmail.com

Received 18 June 2006
Accepted 13 October 2006

Frequent outbreaks of Pichia anomala fungaemia in paediatric patients have warranted the development of a rapid identification system for this organism. This study describes a specific PCR-based method targeting the rRNA gene intergenic spacer region 1 (IGS1) for rapid identification of Pichia anomala isolates and characterization at the strain level. These methods of species identification and strain typing were used on 106 isolates of Pichia anomala (77 from a previously described outbreak and 29 isolated post-outbreak from the same hospital). Using conventional morphological and biochemical methods, 11 strains isolated during the outbreak were misidentified as P. anomala. BLAST analysis of sequences of internal transcribed spacer (ITS) regions of rRNA genes confirmed that they were Pichia guilliermondii (eight isolates) and Debaryomyces hansenii (three isolates). Strain typing of Pichia anomala isolates confirmed the previous finding of a point-source outbreak. The results suggest that IGS sequences and their polymorphisms could be exploited for similar typing methods in other organisms.

Abbreviations: IGS, intergenic spacer region; ITS, internal transcribed spacer.






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