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J Med Microbiol 56 (2007), 177-184; DOI: 10.1099/jmm.0.46775-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Relationship between O-antigen subtypes, bacterial surface structures and O-antigen gene clusters in Escherichia coli O123 strains carrying genes for Shiga toxins and intimin

Lothar Beutin1, Quan Wang2,3, Dieter Naumann4, Weiqing Han2,3, Gladys Krause1, Luciana Leomil5, Lei Wang2,3 and Lu Feng2,3

1 National Reference Laboratory for Escherichia coli, Centre for Infectiology and Pathogen Characterization, Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, D-12277 Berlin, Germany

2 TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, P. R. China

3 Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, P. R. China

4 Robert Koch Institute, P13, Nordufer 20, D-13353 Berlin, Germany

5 Departamento de Microbiologia, Instituto de Ciências Biomédicas II, Universidade de São Paulo, 05508-900, São Paulo, SP, Brazil

Correspondence
Lu Feng
fenglu63{at}nankai.edu.cn

Received 12 June 2006
Accepted 14 September 2006

Escherichia coli O123 strains express a broad spectrum of phenotypes, H serotypes and virulence markers and are able to colonize and to cause disease in different hosts including humans. In this study, two subtypes of E. coli O123 antigen (group I and group II) have been identified based on their cross-reactions with other E. coli O antigens. Investigation of the relationship between O123 group I and group II strains by O serotyping and Fourier transform infrared spectroscopy of whole bacteria revealed surface structural differences between these two groups of E. coli O123 strains. Nucleotide sequence analysis of the O-antigen gene clusters of two E. coli O123 strains representing O123 group I and group II revealed no change at the amino acid level. These findings indicate that the differences in the surface structures of group I and group II strains are not related to genetic heterogeneity in their O-antigen gene clusters. A PCR assay based on O123 antigen-specific wzx and wzy genes was developed and found to be suitable for reliable detection of all subtypes of E. coli O123 strains, which bears an advantage over traditional serological detection.

Abbreviations: AEEC, attaching and effacing Escherichia coli; FT-IR, Fourier transform infrared; STEC, Shiga toxin-producing Escherichia coli.

The GenBank/EMBL/DDBJ accession numbers for the sequences of the E. coli O123 strains 43w and CB9827 O-antigen gene clusters are DQ676933 and DQ676934, respectively.




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