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J Med Microbiol 56 (2007), 1608-1610; DOI: 10.1099/jmm.0.47511-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described real-time PCR targeting the pertactin gene

Karen B. Register and Tracy L. Nicholson

Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, Ames, IA 50010, USA

Correspondence
Karen B. Register
kregiste{at}nadc.ars.usda.gov

Received 13 July 2007
Accepted 18 August 2007

Recently, a real-time PCR (RT-PCR) assay based on sequence from the gene for pertactin was proposed for identification of Bordetella pertussis. Here, it is reported that the B. pertussis pertactin gene sequence for the region that encompasses the RT-PCR probe and primers is nearly identical to that of many Bordetella bronchiseptica strains of human and avian origin. Additionally, it is demonstrated that such strains are erroneously identified as B. pertussis using the RT-PCR assay. These data suggest that the use of the assay without confirmatory testing may result in erroneous identification of a significant proportion of human isolates of B. bronchiseptica as B. pertussis.

Abbreviations: RT-PCR, real-time PCR.

The GenBank/EMBL/DDBJ accession numbers for the Bordetella sequences determined in this paper are EU031919–EU032001.

A table showing B. bronchiseptica isolates used for sequence comparisons is available as supplementary data with the online version of this paper.




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B. Vincart, M. Hallin, M. J. Struelens, and O. Denis
Authors' reply to 'Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described RT-PCR targeting the pertactin gene'
J. Med. Microbiol., March 1, 2008; 57(3): 399 - 400.
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