J Med Microbiol NEW Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rabello, R. F.
Right arrow Articles by Castro, A. C. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rabello, R. F.
Right arrow Articles by Castro, A. C. D.
Agricola
Right arrow Articles by Rabello, R. F.
Right arrow Articles by Castro, A. C. D.
J Med Microbiol 56 (2007), 1505-1511; DOI: 10.1099/jmm.0.47357-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Multilocus sequence typing of Staphylococcus aureus isolates recovered from cows with mastitis in Brazilian dairy herds

Renata F. Rabello1, Beatriz M. Moreira1, Regina M. M. Lopes1, Lúcia M. Teixeira1, Lee W. Riley2 and Angela C. D. Castro1

1 Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

2 School of Public Health, University of California, Berkeley, CA, USA

Correspondence
Angela C. D. Castro
acastro{at}micro.ufrj.br

Received 20 April 2007
Accepted 4 July 2007

Staphylococcus aureus is a major pathogen associated with bovine mastitis, one of the most important infectious diseases occurring in dairy cattle herds worldwide. In the present study, S. aureus isolates recovered from cows with mastitis in dairy herds located in the south-east of Brazil were genotyped by PFGE and multilocus sequence typing (MLST). PFGE identified 60 pulsotypes (PTs), which were found to be distributed among six clonal complexes (CCs) by MLST. All PTs with similarity percentages greater than 65 % belonged to the same CC. Most of the PTs belonged to CC126 (n=28) and CC97 (n=19), which were represented by 91 % of the isolates. These CCs have also been recovered from cows with mastitis in countries located in different continents, but they have rarely been isolated from human specimens. Few isolates were represented by PTs belonging to CCs that are frequently isolated from human specimens (CC1, CC5 and CC30). These data reinforce the hypothesis that a limited number of S. aureus CCs are responsible for most bovine mastitis cases internationally. Specific features of the specialized clones should be studied for use as future targets of mastitis control measures.

Abbreviations: CC, clonal complex; MLST, multilocus sequence typing; MRSA, meticillin-resistant Staphylococcus aureus; PT, pulsotype; ST, sequence type.




This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
D. S. Smyth, E. J. Feil, W. J. Meaney, P. J. Hartigan, T. Tollersrud, J. R. Fitzgerald, M. C. Enright, and C. J. Smyth
Molecular genetic typing reveals further insights into the diversity of animal-associated Staphylococcus aureus
J. Med. Microbiol., October 1, 2009; 58(10): 1343 - 1353.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2007 Society for General Microbiology.