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J Med Microbiol 56 (2007), 1467-1473; DOI: 10.1099/jmm.0.47363-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis

Wataru Yamazaki-Matsune1, Masumi Taguchi1, Kazuko Seto1, Ryuji Kawahara1, Kentaro Kawatsu1, Yuko Kumeda1, Miyoshi Kitazato2, Masafumi Nukina3, Naoaki Misawa4 and Teizo Tsukamoto1,{dagger}

1 Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan

2 Yodogawa Christian Hospital, Osaka, Japan

3 Microbiological Department, Kobe Institute of Health, Hyogo, Japan

4 Department of Veterinary Public Health, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan

Correspondence
Wataru Yamazaki-Matsune
wataru{at}iph.pref.osaka.jp

Received 26 April 2007
Accepted 9 July 2007

A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.

{dagger}Present address: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA sequences are AB301955–AB301967.




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