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J Med Microbiol 56 (2007), 1460-1466; DOI: 10.1099/jmm.0.47322-0
© 2007 Society for General Microbiology
ISSN 1473-5644

Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries

Seon Young Choi1,2, Yoon-Seong Jeon1,3, Je Hee Lee1,2, Boram Choi1, Sun Hwa Moon1, Lorenz von Seidlein1, John D. Clemens1, Gordon Dougan4, John Wain4, Jun Yu4, Je Chul Lee5, Sung Yong Seol5, Bok Kwon Lee6, Jae-Hoon Song7, Manki Song1, Cecil Czerkinsky1, Jongsik Chun1,2,3 and Dong Wook Kim1

1 International Vaccine Institute, San 4-8 Bongcheon 7 dong, Kwanak gu, Seoul 151-818, Republic of Korea

2 School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea

3 Interdisciplinary Program of Bioinformatics, Seoul National University, Seoul 151-742, Republic of Korea

4 The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK

5 School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea

6 Laboratory of Enteric Infections, Department of Microbiology, National Institute of Health Korea, Republic of Korea

7 Division of Infectious Disease, Samsung Medical Center, Seoul, Republic of Korea

Correspondence
Dong Wook Kim
dwkim{at}ivi.int

Received 30 March 2007
Accepted 6 July 2007

The multilocus sequence typing scheme used previously for phylogenetic analysis of Escherichia coli was applied to 107 clinical isolates of Shigella flexneri. DNA sequencing of 3423 bp throughout seven housekeeping genes identified eight new allele types and ten new sequence types among the isolates. S. flexneri serotypes 1–5, X and Y were clustered together in a group containing many allelic variants while serotype 6 formed a distinct group, as previously established.

Abbreviations: EIEC, enteroinvasive E. coli; MLST, multilocus sequence typing; ST, sequence type.

The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences of the new allele types identified in this study are EF364101–EF364108 (icd123, EF364101; adk116, EF364102; fumC149, EF364103; fumC145, EF364104; purA98, EF364105; purA96, EF364106; purA97, EF364107; purA95, EF364108).

A full list of clinical S. flexneri isolates subjected to MLST analysis in this study and a phylogenetic tree generated by the neighbour-joining method for S. flexneri are available as supplementary material with the online version of this paper.






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